It is difficult to define the detection limits obtained by chelation - solvent extraction 确定螯合- -溶剂萃取法所得到的检测限颇为困难。
2.
Ac - hac is a kind of polymer , which has strong chelation ability with cd2 + because of its carboxyl group 本方法得到的cds纳米粒子比前一种方法得到的cds纳米粒子具有更好的发光性能。
3.
The expressed proteins were purified by ni2 + chelation affinity chromatography . the recombinant proteins were prepared with the purity more than 95 % 用ni ~ nta亲和层析柱进行纯化，获得1 ’ rka膜外域各结构域重组蛋白，纯度均在95 %以上。
4.
The chelation ability made ac - hac act as a protection layer on the surface of cds nanoparticles , which reduced surface states and improved the band edge emission . 3 这是因为聚丙烯酸?丙烯酸羟丙酯在一定程度上能够修饰cds纳米粒子表面，减少表面缺陷，提高纳米粒子的带隙发光。
5.
The expressed products were purified by metal ( ni2 + ) chelation affinity chromatography and tested by western - blot . the results showed that his - tagged vp6 fusion proteins have a positive reaction with anti - jl94 serum 采用western - blot对表达产物及纯化产物进行反应原性分析，结果表明所得his - taggedvp6融合蛋白具有较好的抗原反应特异性。
6.
Then the product was purified by glutathione sepharose 4b chelation affinity chromatography , and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application 表达产物经谷胱甘肽琼脂糖4b亲和层析纯化后，得到了纯度较高的gst - gnrh trs融合蛋白。为进一步科研和实际应用奠定基础。
7.
Gfral was high expressed in e . coli after iptg induction . by ni2 + chelation affinity chromatography , the purified recombinant gfral protein were obtained . based on the evolutionary trace method , multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family 2 . gdnf的表达及其对pc12基因工程细胞的影响用本教研室已构建好的表达质粒pet一gdnf转化大肠杆菌bl21 ( de3 ) ，经lmmipto诱导gdnf表达，并在niz气nta柱上进行纯化，稀释复性后，纯度达90 %以上。
8.
Both the frequency and intensity of 1146 . 0cm - 1 change largely , etc . discussed and compared the mechanism of these interaction in detail we got some conclusions as following : both zn2 + and cd2 + ions can chelate with po2 - and come into being - po2 - me2 + n7 ( purine ) chelate . as to zn2 + ions , when r2 . 0 , the quantity of po2 - zn2 + n7 is directly linear with r . when r2 . 0 , zn2 + ions even can chelate with c at n3 - o2 sites and this chelation will disrupt the h - bond between gc base pairs and make the dna structure unstable . as to cd2 + ions , only when r is within 1 . 0 - 1 . 5 can this chelation take place and the relation of the quantity of this chelate with r is not easy to conclude 通过对这些变化进行详细讨论和分析，我们得到了如下的结论： zn ~ ( 2 + )和cd ~ ( 2 + )都可以跟dna的磷酸基团螯合，形成- po _ 2 ~ - … me ~ ( 2 + ) … n7 ( g )螯合物，但是前者的螯合跟离子浓度有很大的关系，在摩尔浓度比小于2 . 0时，螯合物的生成量跟r成正比关系，而后者的螯合跟离子的关系不是很明显，只有当r处于1 . 0至1 . 5之间时，螯合作用才能进行，并且螯合物的生成量跟r之间没有很明显的关系存在。
9.
This paper reviews the effects of al on photosynthesis , photoprotective systems , water use efficiency ( wue ) , water content , carbohydrate content , mineral nutrition , organic acids , and nitrogen metabolism in plant shoot , and also the mechanisms of al detoxification ( chelation of al with small organic compounds , and isolating al in the some parts , which are insensitive to al , such as vacuoles or epidermal cells ) by al accumulators 为此，本文综述了铝对植物地上部光合作用、光保护系统、水分利用效率、含水量、碳水化合物含量、矿质营养、有机酸和氮代谢的影响，并对富铝植物的解铝毒机制（铝与小分子有机酸螯合和把铝隔离在对铝不敏感的表皮细胞和液泡内）进行了综述。
10.
These mechanisms involve in the binding of heavy metals to cell wall , immobilization exclusion of the plasma membrane , efflux of these toxic metal ions , reduction of heavy metal transport , compartmentalization and metal chelation by tonoplast - located transporters , expression of more general stress response mechanisms such as stress proteins 这些机制包括重金属离子与细胞壁的结合，限制重金属离子透过细胞膜，重金属离子或化合物的排出，重金属在植物体内的转移，重金属的络合与区隔化以及植物对胁迫的基本的反应机制如胁迫蛋白含量的变化等。
