And maxi chimerism rate is 6 % estimated from fish results of 32 bacs 对32个bac克隆的荧光原位杂交结果表明，文库的最大嵌合率为6 。
2.
Hematopoietic chimerism produces durable and robust immune tolerance to kidney allografts , although incomplete tolerance to donor skin grafting 造血嵌合能够对肾移植产生持久有力的免疫耐受，但对于皮肤移植无效。
3.
These data showed that the chimerism were specially tolerant to donor xenoantigens in skin grafting and mixed lymphocyte reaction ( mlr ) assays , however were still immunocompetent to mhc - disparate third party c57bl / 6 mouse . in the second experiment , mice of group b appeared with variant degree of wasting , diarrhea , fur losing , and all died within 15 days . pathologic changes of liver and intestine were inflamming cells infiltrating and necrotizing 肝、小肠切片均有明显病理改变，主要表现为肠粘膜部分脱落、坏死和炎性细胞浸润，肝实质内有灶性淋巴细胞及多形细胞浸润，并可见局灶性坏死，两者均符合gvhd病理改变。
4.
Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras . the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02 , has enough polymerism and good stability , and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line , especially when involving strains that can not be discerned with coat color or biochemical markers 尝试应用多位点dna指纹技术，检测经过胚胎干细胞es细胞途径所获得的嵌合体小鼠中es细胞在各种脏器中的嵌合情况检测es细胞在嵌合体小鼠中是否实现种系传递。结果表明：采用新型的人工合成的寡核苷酸多聚体探针jl - 02探针的多位点dna指纹图谱，具有足够的多态性和很好的稳定性。
5.
This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability , then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection . finally , first chimeric pig in china was delivered , and by chimera analysis , chimerism is present in some tissues 本研究首先利用近交系昆明白小鼠进行胚胎显微注射观察发育效果，然后利用所获五指山猪eg细胞制作嵌合体检验eg细胞多能性，最终获得国内首例胚胎干细胞( eg细胞)嵌合体猪，经dna分析确认在多种组织中存在嵌合状态。
6.
The experiment includes two procedure . first , sd rats were conditioned with 5 . 5gy sublethal total body irradiation ( tbi ) , followed by infusion of balb / c mice bone marrow cells 8x107 on day 0 , then were intraperitonially administered cyclophosphamide ( cp ) 150mg / kg on day 2 . the procedure let sd rats become chimerism and have specific immunologic tolerance 实验分两步：第一步， sd大鼠经亚至死剂量5 . 5gy全身照射后，尾静脉输入balb c小鼠的骨髓细胞8 10 ~ 7 ， 2天后腹腔内注射环磷酰胺150mg kg ，诱导形成嵌合体大鼠，使其对balb c小鼠产生特异性免疫耐受。
7.
After dna analysis , six white pigs is not present chimerism in various tissues . to one skin pigment piglet , chimerism is present in skin , brain , pancreas , pituitary gland and blood , but not in heart , lung , liver , spleen , kidney , intestine and ovary . the detection of another skin - pigment chimera is similar to the analysis result of wzsp 通过dna分析进一步得出，所产8头仔猪，其中六头白猪体组织未发现嵌合现象；其中一头皮肤色素嵌合体猪的皮肤、大脑、胰、甲状腺、血是嵌合的，而心、肺、肝、脾、肾、空肠、卵巢这些组织并没有嵌合；另一头皮肤色素嵌合体猪的检测结果和五指山猪取得相似的结果。
8.
In summary , we constructed and characterized a bac library of silky , a unique chinese native breed of chicken . the library has high genome coverage ( 13 - fold ) , chimerism ( 6 % ) and overlapped bacs , which made it a valuable resource to complete chicken physical map , study functional genes and construct bac contigs . we analyzed the whole genomic sequence and snps of tyrp1 and found that silky tyrp1 is different with other breeds both in microsatellite and transcription regulation site 中国农业人学博卜学位论文摘要本研究构建了中国特有鸡种丝羽鸟骨鸡基因组bac文库并进行了文库质量鉴定，它所具有的13倍高基因组覆盖率、 6 %的嵌合率和部分重叠的克隆使其成为完善鸡的基因组图谱、研究基因功能和构建bac重叠群的优质资源。
9.
Different amount of copies in different tissues attribute to the different density of positive signals . the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome . and because the exogenous dna occurs losing the segments . partly integration , or existin g outside of genome dna , the rate of chimerism is relatively high 结果表明： ( 1 )脂质体包裹外源基因转染精子的方法，可将外源基因导入受精卵中，能够获得转基因动物，并得到了较高的转基因阳性率； ( 2 )精子携带的外源dna的整合过程是随机的，在受精过程和胚胎早期分化过程中可能发生了片段丢失、不完全整合或游离于基因组存在而产生嵌合体。
