In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli 根据已发表的iltvtk基因的核苷酸序列设计一对pcr引物，以增殖的两株iltv的dna为模板，分别对它们的tk基因进行pcr扩增。将回收的pcr产物连接到适当的质粒载体上，转化感受态大肠杆菌，通过筛选对iltvtk基因的阳性克隆进行扩增培养。
