| 1. | Preventive effect of ifn - receptor - immunoglobulin fusion protein on liver demages induced by cona in mice
诱导小鼠肝损伤的保护作用 |
| 2. | Basing on these backgrounds and motives , the cona expressing library of plasmodium falciparum from hainan was constructed
正是基于以上目的和研究背景，我们构建了恶性疟原虫海南株红内期cdna表达文库。 |
| 3. | After activated by cona , the mouse spleen t lymphocytes started expressing baf complex and nf1 / ctf . the longer the stimulation , the higher the expression level of baf complex and nf1 / ctf in the cells
经cona激活后，小鼠脾t淋巴细胞中baf复合物和转录因子nf1 ctf开始表达，随着刺激时间的增加，表达量增加。 |
| 4. | Further , in the mouse spleen t lymphocytes activation system , it was found that , without activation by cona , there were no or extremely low level of expression of baf complex and nf1 / ctf in the spleen t lymphocytes
进一步用小鼠t淋巴细胞活化体系的研究发现，未经cona激活的t淋巴细胞中， baf复合物和转录因子nf1 ctf不表达或低水平表达。 |
| 5. | In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 |
| 6. | According to published chil - 15 gene sequence , a pair of primers were designed and synthesized to clone chil - 15 cdna from cona - activated chicken splenocytes by rt - pcr . recombinant plasmid puc19chil - 15 carrying the chil - 15 gene was constructed
鸡il - 15 ( chil - 15 )是一种新发现的且与il - 2活性相似的细胞因子，作为疫苗佐剂研究的重要候选细胞因子有巨大潜力。 |
| 7. | Fty were also found to inhibit the expression of cd25 on t cells stimulated by cona . conclusion : we assumed tolerance to allograft was induced by and only by the pretreatment of fty in this stuty
结论：我们的实验完整观察了fty对淋巴细胞及t细胞亚群的影响，验证了fty可诱导淋巴细胞凋亡，首次发现fty对t细胞早期活化反应的抑制作用，其中包括减少cd25的表达。 |
| 8. | Interferon - is an antiviral cytokine produced by activated t cell and nk cell . it upregulated mhc ii antigen expression , influenced the cellular and humoral immune response and has pleiotropic regulatory effects on immune system . one gene was amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) in splenocytes which was stimulated with cona for 24 hours
-干扰素( interferon - ， ifn - )主要由活化的t细胞及nk细胞产生，具有抗病毒作用，也具有上调mhc ( majorhistocompatibilitycomplex , mhc )类抗原分子的表达等作用，是影响机体细胞免疫和体液免疫反应的一类具有多种调节效应的细胞因子。 |
| 9. | In this study , we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene . about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next , we construct recombinant plasmid pproex ? t - il - 2 . the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t - il - 2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il - 2 protein
经酶切鉴定及dna序列测定，该基因为鸡il - 2基因，其序列与sundick等报道的完全一致。在此基础上，我们把鸡il - 2基因亚克隆到大肠杆菌原核表达载体pproex ~ ( tm ) ht中，构建重组表达质粒并进行确证性序列测定，重组质粒测序结果表明将编码鸡il - 2成熟蛋白的基因正确地插入到原核表达载体pproex ~ ( tm ) ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养， sds - page和westernblot分析显示，表达的鸡il - 2融合蛋白分子量约为18kda ，表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30 。 |
| 10. | 3 . the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme , the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona . the results showed that lymphocyte proliferation induced by pha was better than by cona . 4
3 、 gpif免疫生物活性评价方法的优化对gpife玫瑰花环实验在不影响srbc活性的基础上用神经氨酸酶处理，经此法处理后e玫瑰花环形成率明显提高；对传统的cona诱导的人外周血淋巴细胞增殖brdu - elisa实验，进行了方法学改进。 |
