It is efficient for flow cytometric analysis when fresh platelet riched plasma ( fprp ) was set as negative control , thrombin actived fprp and liquid nitrogen treated fprp were set as positive control respectivelv 除了fcm常用的同型对照外，文中还将fpr 、凝血酶激活fprp和液氮处理fprp作为血小板标准阴性、阳性对照，直接应用于fcm分析，且效果良好。
2.
Section 2 flow cytometry combined assay for phosphatidylserine and cd62pexpressed by preserved platelets human platelets have distinct characters when preserved by different methods . a efficient cytometric assay for different preserved platelets expression of cd62p and phosphatidylserine ( ps ) is in dire need 第二节，流式细胞术联合测定保存血小板表达的磷脂酞丝氨圈和cd62p人类血小板通过不同的技术进行离体保存后，其特性改变差异较大。
3.
( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis . we found condensed , marginating , half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis ( 2 )二氧化硫吸入可引起小鼠脾脏细胞出现明显的凋亡改变，红髓区和白髓区淋巴细胞出现核固缩，染色质凝聚、边集； dna凝胶电泳分析发现168mg m ~ 3二氧化硫染毒组出现典型的dna梯形条带；流式细胞分析也发现高剂量染毒组的小鼠脾细胞凋亡率增加，并且与对照组相比有显著性差异， p 0 . 05 。
4.
This paper is the working results of flow cytometric measurements of ultraphytoplankton in east china sea and yellow sea , which including the works of two cruises in autumn 2000 and spring 2001 and two - week time series observation in summer 2001 . in addition , in situ enrichment experiments were designed at e2 station of spring cruise 本文主要内容为流式细胞计在中国东、黄海对超微型浮游植物的检测结果报导，所做工作包括： 2000年秋季及2001年春季的两个航次、 2001年夏季( 7月)在长江口区的定点长时观测，此外，还在春季航次e2站进行了现场营养盐加富培养实验。
5.
The zones of coexisting distribution can be influenced by hydrological conditions . in this paper , a method is used to estimate the biomass contribution of per population to total biomass that combined flow cytometric analyzing data and size - fractionated chlorophyll a data . the results of autumn cruise shows that synechococcus is a important contributor to pico - chlorophyll a , especially in e2 , e6 , e7 also be true to total chlorophyll a , which the percent value is 69 % , 56 % , 56 % respectively 本文还尝试把流式细胞计分析的数据与叶绿素a分级数据结合，粗略估计微微型浮游植物各类型的叶绿素a贡献，对秋季结果分析表明，在秋季聚球藻是pico一叶绿素a的主要贡献者，在e2 、 e7 、 e6站甚至是总叶绿素a的主要贡献者，分别达到69 % 、 56 % 、 56 % ，不过本法还存在许多缺陷，有待于进一步探讨。
6.
Normal bn rats were vaccinated intraperitoneally with lctcv , ltcv and rpmi1640 culture buffer ( blank control ) respectively . one - way mixed lymphocyte reaction ( mlr ) , flow cytometric analysis of the apoptosis of peripheral t cell and the rate of cd4 / cd8 in peripheral blood were performed before and after vaccination . results mlr showed that the immune response capability of bn rat spleen cells in lctcv group were suppressed significantly after vaccination ( p < 0 . 01 ) 于疫苗接种前和每次接种后第5天以被免疫bn大鼠的脾细胞作为反应细胞，以lou c大鼠的脾细胞作为刺激细胞(经丝裂霉素处理) ，进行单向混合淋巴细胞反应( mlr ) ，同时对外周血t细胞凋亡和cd _ 4 ~ + 、 cd _ 8 ~ + t细胞亚群进行流式细胞分析。
7.
It has marked apoptotic induction on mouse spleen cells tested by dna agarose gel electrophoresis analysis and flow cytometric assay in certain degree . it also can decrease the activity of lactate dehydrogenase ( ldh ) outside of mouse the spleen cells and increase the quantity of reduced gsh inside the mouse spleen cells 而且一定浓度范围内可引起小鼠脾淋巴细胞dna发生特征性降解，出现dnaladders ，引起染毒组细胞凋亡率显著性升高，但细胞凋亡率与剂量不呈直线相关关系。
8.
Gly - pro - arg - pro acetate salt ( gprp ) was applied to prevent platelets aggregation and fibrin formation , stabilize platelets and minimize the artifical platelets activation . this is also the key to conquer difficulty of flow cytometric quantitive analysis when platelet , ca2 + and plasma coexist . this flow cytometric method is specially suitable for the multi - param 该方法中glypro argpro乙酸盐（ gp肝）的选择应用，既防止了血小板聚集和纤维蛋白的形成，同时可稳定血小板，减少制备过程中人为激活，克服了在血小板、 c吓血浆共存条件下fcm定量分析血小板的困难，尤其是为深低温处理血小板包括ps在内的多参数分析提供了良好的方法基础。
