| 1. | Cotton transformation based on inverted repeat construct of oleic acid desaturase gene ghfad
1倒位重复构建的遗传转化研究 |
| 2. | Heteroexpression of rhizopus arrhizus 6 - fatty acid desaturase gene in pichia pastoris
脂肪酸脱氢酶基因在毕赤酵母中的表达 |
| 3. | Effect of over - expression of sterol c - 22 desaturase on ergosterol production in yeast strains
22去饱和酶高表达对酵母细胞麦角甾醇合成的影响 |
| 4. | The coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into vector pbi121 containing intron - kanamycin
再生苗经过戍r和pcrs 。 : : hernbl 。 t分子检测证明获得了烟草转基因植株。 |
| 5. | A prokaryotic expression vector was succsessfully constructed and transformed in to e . coli expression strain bl21 ( de3 ) . with the iptg induction , a 50kd protein of - 6 fatty acid desaturase is expressed
原核表达载体petfad2转化到表达菌株bl21 ( de3 )后，经iptg诱导得到了目标蛋白。 |
| 6. | Northern blot analysis showed higher expression level of pds gene in the stigma and anther than in the leaf and stem . the information we have got is very important for the further research of gene structure , expression and function of phytoene desaturase
此基因的克隆，为深入研究八氢番茄红素脱氢酶基因结构、表达和调节机制及其结构和功能的关系奠定了重要的基础。 |
| 7. | Sequence analysis showed highly similarity to other plants such as narcissus pseudonarcissus . , and they all possess a short highly conserved n - terminal sequence encoding a dinucleotide cofactor binding site . thus it is a new member of phytoene desaturase gene family
由此可认为该番红花八氢番茄红素脱氢酶基因在结构上与其它植物八氢番茄红素脱氢酶基因是同源的，是八氢番茄红素脱氢酶基因家族中的一个新成员。 |
| 8. | Phytoene desaturase ( pds ) has recently been identified as an important enzyme in carotenoid metabolitic pathway . a new full - length cdna clone encoding phytoene desaturase gene was isolated from stigma of saffron using rt - pcr and race techniques ( ( genbank accession no . ay 183118 ) )
其中主要的活性成分是西红花甙，需经类胡萝卜素代谢途径合成，而八氢番茄红素脱氢酶( phytoenedesaturase )是此途径中的一个早期关键酶。 |
| 9. | For prokaryotic expression , the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ) . the gene was overexpressed in e . coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction
用pcr方法扩增番红花八氢番茄红素脱氢酶( pds )编码区序列，定向克隆到表达载体pet一艺1a ( + )上，获得重组质粒pet一21a ( + ) / cspds 。 |
| 10. | On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection
本实验以pegfp - n1质粒为骨架载体，用酶切连接的方法构建一个顺序含有- actin启动子、 fad2cdna 、 sv40polya加尾信号的真核表达载体，双切线性化后回收，使用回收的表达载体经原核显微注射生产转基因小鼠。 |
