It has been 40 years since dobereiner and ruschel isolated the nitrogen - fixing bacteria from the rhizosphere of sugarcance plants and demonstrated the potential of diazotrophs to associate with graminaceous plants . more recent evidence of significant biological nitrogen fixation in economical important graminous species , particularly sugar cane , rice and forage grasses , has induced tremendous interest in ni fixation by non - legumes 本研究分离、筛选得到一株固氮酶活性高且稳定，生长势强的联合固氮菌株，并对其分类地位、形态及生理特征、对环境的适应性及其对植物的促生效果和作用机理作了系统研究，得结果如下。
