Except the igy got from by the method 6 % octanoic acid combined with ammonium sulfate , there were over two tape in others sds - page electrophoretogram 获得igy产量以海藻酸钠?硫酸铵法最高，可达5 . 69mg ml ,其次是用3辛酸?硫酸铵和聚乙二醇法。
2.
The results demonstrated that the activities of pod isoenzymes increased under the sound . compared with control group , there was no new enzyme band in pod electrophoretogram for stimulation groups 结果表明刺激组与未经声波处理的对照组相比， g0 / g1期细胞减少， s期细胞增多，说明声波刺激促进了细胞的生长，有助于细胞有丝分裂。
3.
The results showed that the electrophoretogram patterns of isozymes , serum and muscle protein of bumper crucian carp were the same as those of its female parent and significantly different from its male parent . it is speculated that the function of heterogeneous sperm was only in dna level and not behave on the isozyme expression of grown progeny 结果发现：异源子代的血清、肌肉蛋白和同工酶的电泳图谱与母本彭泽鲫相同而与父本海鲤显著不同，推测异源精子的作用可能是在dna水平上对后代产生影响，这种影响没有表现在成体同工酶的表达上。
4.
Secondly , analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation . it could be seen from electrophoresis gel that each group had 6 enzyme bands . new enzyme band in pod electrophoretogram was n ' t detected for stressed groups 此外，在部分实验组的培养基中加入不同浓度的蛋白质合成抑制剂环己亚胺酮（ chm ）后发现， pod和cat的活性有所降低，暗示着声波处理使保护酶活性升高的原因可能是声波处理促进了细胞内酶的合成。
5.
Isozymes in different tissues of three stains of crucian carp were studied comparatively . the results indicated that pengze crucian carp is different from silver crucian carp in biochemical level , they probably came from different region . the electrophoretogram of pengze crucian carp and silver crucian carp all included the basal patterns of wild crucian carp 通过对三种鲫鱼品系不同组织同工酶的电泳图谱的比较研究，揭示了彭泽鲫和银鲫至少在生化水平上已有明显的分化，很可能起源于不同的地区，独立演化而形成。
