3 . successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0 . 6mol / lnacl solution as an eluant 3 .采用阴离子树脂deae一纤维素对透明质酸粗品进行纯化，当naci为0 . 6mol / l时，蛋白质含量较低。
2.
To reveal the reason of above phenomenon , we purified the eluant by poros 20hq ion - exchange chromatography and got five proteins . one of them ( hq - s ) was proved to can be proteolytic 为弄清这一现象的产生原因，将嗜硫色谱洗脱峰通过poros20hq离子交换色谱进一步分离，获得5个分子量非常接近的组分。
3.
When it was verified to be pure by sds - page , page and clc , it could be concluded that hq - 5 ' s autoproteolysis just was the cause of the proteolytic phenomenon of eluant of the thiophilic adsorption chromatography hq - 5 was identified as a glycoprotein containing 3 . 5 % neutral carbohydrates 由此可断定嗜硫色谱洗脱峰的降解现象是由hq - 5的自降解引起的。 hq - 5经sds - page电泳测得分子量为47 . 6kda ，含有链内二硫键，加dtt处理后，分子量为50 . 8kda 。
