| 1. | 7 . we found ent - kaurene oxdase belong to a multigene family by southern blot . we deduced that multigene response to different development signal
这预示着此酶在植物生长发育过程中起着多种作用，不同的基因可以响应不同的发育信号。 |
| 2. | The full length of the strawberry ent - kaurene oxidase has 1650 bp , and code region has 539aa . the similarity among three rose family specy are 65 % , 79 % , 57 % . 2
苹果，草莓和桃3种蔷薇科植物的贝壳杉烯氧化酶的序列同源性分别为65 ， 79 ， 57 ， 58 。 |
| 3. | In this article we cloned two crucial gibberellins biosynthesis enzyme genes : ent - kaurene oxidase and 20 - oxidase , and we investigated their expression patterns . 1
本文克隆了赤霉素生物合成途径中关键合成酶：贝壳杉烯氧化酶和20 -氧化酶，并研究了二者在果树生长发育中的表达规律。 |
| 4. | By investigate the primary structure of strawberry ent - kaurene oxidase , we found that it has four conserve amino acid sequence . it suggested that these conserved regions are important in gene function
对一级结构分析发现4段非常保守的区域，推测是在此酶行使功能时的重要组成部分。 |
| 5. | We cloned two ent - kaurene oxidase homologues from the reverse transcriptional products of different strawberry tissue , the identity of these two homologues is 88 . 2 % . it suggest us that it belongs to a gene family . 3
在丰香草莓的不同发育部位克隆到同源性为88 . 2的两个贝壳杉烯氧化酶的基因片段，预示着此基因是个基因家族。 |
| 6. | We designed degenerate primers of consensus amino acid sequences of ent - kaurene oxdase , and use them to amply the middle segments of apple , strawberry and peach , and then we designed specific primers insides these middle segments to amplify 3 " termini , and 5 " termini . finally we got the full length of strawberry and 3 ' teminal of peach
利用保守区域的兼并引物克隆了平邑甜茶，丰香草莓和碧桃贝壳杉烯氧化酶基因的长为410bp的中间片段，以中间片段序列为线索又克隆到萆莓贝壳杉烯氧化酶的全长，和碧桃此酶的3 ’端。 |
| 7. | 4 . we use half quantification and digoxigenin detection to investigate the expression pattern of ent - kaurene oxidase . we found that it was regulated by development , it expressed high in the fast growing tissue , expanded leaves under a fast growing circumstance also express high level of ent - kaurene oxidase
利用半定量rt - pcr的方法结合地高辛标记法，贝壳杉烯氧化酶在果树中的表达是受发育调控的，其在旺盛生长的组织中表达量高，在植株旺长期的成年叶中表达量也很高。 |
