The construction of eucaryote expression vector was accomplished , and the prophase preparation for eucaryote expression of halr gene was made 并完成了真核表达载体的构建，为halr基因的真核表达做好了前期准备。
2.
The object of this study is halr , focus on its molecular cloning , construction of eucaryote expression vector and procaryotic expression 本课题选用halr作为研究对象，重点研究其分子克隆、真核表达载体构建及原核表达。
3.
Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ) , then subcloned into the eucaryote expression vector pcdna3 . 1 ( + ) after sequence analysis 将halr基因克隆到原核融合表达载体pet28a ( + )上，序列分析后亚克隆到真核表达载体pcdan3 . 1 ( + )上。
4.
Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ) . gpi - anchored proteins are synthesized on membrane - bound ribosomes . upon translocation of the pro - protein across the endoplasmic reticulum membrane , gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid Gpi化前体蛋白在依附于膜的核糖体上合成，当其易位穿过内质网( er )膜后，被gpi ：蛋白质转酰胺基酶( gpit )识别， gpit在移走其羧基端gpi信号序列的同时将gpi分子连接至新生成的氨基酸位点上。
5.
Depending on cellular location and function of thioredoxins , there are two thioredoxin systems in high plants , one is nadp / thioredoxin system mainly existing in chloroplast of high plant and eucaryote algae and oxygenic photosynthetic prokaryote , which is composed of nadph , nadph - dependent thioredoxin reductase , thioredoxin - f and thioredoxin - m 在高等植物中，存在两个与硫氧还蛋白密切相关的硫氧还蛋白系统：一个是位于叶绿体中的铁氧还蛋白-硫氧还蛋白系统，该系统中含有两类核基因编码的硫氧还蛋白即f -型trx和m -型trx 。
6.
Thioredoxins , an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc - , are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda . by its disulfide / dithiol interchange reaction , this protein can transmit the regulatory signals to seleted targets ( enzymes , transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis , dna synthesis , transcription , protein disulfide reduction , protein repair , filamentous phage assembly , cell apoptosis and seeds germinating and so on 该蛋白质中含有保守的- cp ( g ) pc -氨基酸活性基序，该基序中的两个半胱氨酸残基可通过巯基二硫键的转换实现其氧化还原状态的变化和电子氢的传递，对细胞中与氧化还原相关的多种生理过程的调节起重要作用。通过同许多酶类、蛋白类、细胞内活性因子相藕连， trx能对光合作用、 dna复制、基因转录、细胞凋亡和生长、噬菌体组装、蛋白质的还原和修复信号传导等生理过程产生影响和调节。
