| 1. | Cloning and prokaryotic expression of 3ab gene of fmdv
基因的克隆与原核表达 |
| 2. | Foot - and - mouth disease virus ( fmdv ) is the etiological agent of an important disease of livestock
口蹄疫是当今世界危害最严重的家畜传染病之一。 |
| 3. | As a result , developing a safe and effective fmdv vaccine has become a research priority
目前研究人员通过基因工程方法开发口蹄疫安全的新型疫苗成为研究的热点。 |
| 4. | The structural protein vp1 of fmdv carries critical epitopes which can induce neutralizing antibodies
口蹄疫病毒的主要抗原组分为vp1结构蛋白，它能够诱导机体产生保护性的抗体反应。 |
| 5. | The sequencing results show that the fmdv - vp1 has mutated compared with the published sequences . and at the same time the 3 dimension protein structure was built for research
通过与genebank中所有的vp1序列进行比较我们发现vp1第24 、 33 、 204位氨基酸突变幅度最大。 |
| 6. | Moreover , mice feeding on fmdv - vp1 transgenic plant leaves in their diet produced a virus - specific immune response that could protect the immunized mice against fmdv challenge
另外利用转基因植物表达口蹄疫病毒vp1抗原蛋白具有安全廉价、蛋白折叠充分、免疫原性良好等特点。 |
| 7. | This study is to express fmdv - vp1 protein using the expression system and analysize the protein structure . in this study bioinformatic method is also used to research the mutation and epidemiology principles of the fmdv
口蹄疫是口蹄疫病毒( footandmouthdiseasevirus , fmdv )感染引起的偶蹄动物的急性、热性、高度接触性传染病。 |
| 8. | The use of transgenic plants for producing fmdv vp1 antigen protein has been reported and may prove to be safer than conventional vaccines . recently , fmdv vp1 protein had be expressed in transgenic plants
最近利用转基因植物表达的口蹄疫病毒vp1蛋白注射和饲喂小鼠后，能够诱导其体内产生特异性中和抗体，并使小鼠得到有效的免疫保护。 |
| 9. | Third , the cloned fmdv - vp1 gene was subcloned into an eukaryotic expression vector , psupery - vp1 . to evaluate the immune responses of this construct , animals sera were used to analyzed their specific antibodies against vp1 using western blot analysis
第四， dna疫苗因其制备简单、使用和保存便利，能构诱导机体产生体液和细胞免疫等优点，成为新一代疫苗研究的热点。 |
| 10. | On our conjecture , gene 3a , a highly conserved sequence , may be the determinant of the virulence of fmdv . changes in 3a have been associated with altered host range . a deletion in 3a has been associated with bovine attenuation of fmdv
我们根据已有资料推测， 3a基因可能是口蹄疫病毒的毒力决定簇，它具有高度保守性，其变异和部分缺失对病毒在牛体内的致弱和宿主嗜性发生改变起重要作用。 |
