N linked glycan processing glucosidases and mannosidasesn 连接聚糖链加工中的葡萄糖苷酶和甘露糖苷酶
2.
The complex of glycan structure is synthesized by glycosyltransferases and the differential expression of glycans during development is due to the developmental expression and activity of glycosyltransferases 复杂的糖链结构是由糖基转移酶依此加工合成的，糖基转移酶在发育过程中的表达水平及生物活性决定了某一特定糖链在发育中的表达变化。
3.
Glycoprotein bands recovered were detected by gelcodeglycoprotein staining kit , and the result showed that recovery rate reached 50 percent , that the quantity of glycoprotein once purified from sds - gel , was enough to be used in ms , hplc , protein sequence analysis , glycan analysis , and characterization so on 从sds胶上纯化的茶树叶糖肽鉴定结果看，茶树叶糖蛋白的sds胶上纯化回收率达50左右。
4.
Part ii effects of gnt - v on the phosphorylation and activity of egfr signaling molecules in h7721 cells modification of the n - glycan structure and function of egfr by the transfection of different gnt - v cdnas may lead to the alterations of the phosphorylation and activity in the downstream molecules of signal transduction Gntv一s / h7721细胞p42 / 44mapk的磷酸化也较mock细胞增强，而gntv一as舰7721细胞则减弱，表明egfr的这两条信号通路中信号分子的磷酸化或活力都受gnt一v的影响。
5.
These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr . it seems that the increased 1 , 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n , while the decrease of this branch may prevent these processes 用特异性抗体结合westemblot结果发现，正义或反义gnt一vcdna的转染并不引起pkb 、 p44 / 42mapk和mek蛋白质表达的变化，而gntv一s / h ” 21细胞pkbt308 、 5473位点磷酸化和免疫沉淀pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化为指标的pkb的活性都较mock细胞增加， gntv一as / h7721细胞中这些指标的变化则相反。
6.
Therefore , the structural modification of n - glycan and the functional changes of egf receptor ( egfr ) in different transfected cells were investigated . it was found that the 1 , 6 glcnac branch on the n - glycans of immuno - precipitated egfr on gntv - s / h7721 cells was increased while it was reduced in gntv - as / h7721 cell despite the unaltered expression of egfr protein 因此本文对不同转染细胞egf受体( egfr )的糖链结构及功能的改变作进一步研究，结果发现，不同转染细胞中，免疫沉淀egfr的蛋白量没有改变，但gntv - s h7721细胞egfr上n -糖链中1 ， 6glcnac分支比mock细胞增多，而gntv - as h7721细胞相反。
