The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation , the specificity of the antibody and the choice of a suitable enzyme label 酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
2.
A competitive binding assay used to determine hapten iaa with the aid of hrp - iaa conjugate has been developed . it was based on the ordered , stable mercapto self - assembled monolayer formed on gold surface , which immobilized igg with covalent bond 方法基于金基底上形成的均一、稳定、有序的巯基乙酸自组装单层膜，在edc 、 nhs活化下，能以共价方式固定抗体。
3.
The protein accounting for the total was approximately 11 . 80 % . the peptide was synthesized according to the sequence of grb - ast7 . since ast7 is a hapten , it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc ) 根据ast _ 7氨基酸序列合成小肽，用edc做偶联剂把合成的半抗原小肽与载体bsa偶联成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa测定效价为1 400 。
