It is misspent time to calculate and report hemagglutinating titers for cell hybrid studies . 在细胞杂交过程中计算和报道血球凝集的滴定度是浪费时间。
2.
The effects of metal ions and denaturants on the conformation and hemagglutinating activity of crotalaria mucronata lectin 金属离子和变性条件对野花生豆凝集素活性和构象的影响
3.
The hemagglutinating characteristic of jl94 was studied tentatively with human and 9 kinds of animal red blood cell ( rbc ) 采集人及9种动物的红细胞,用病毒的细胞培养物做血凝和血凝抑制试验,来检测jl94的血凝特性。
4.
The hemagglutinating activity of the fluids was not affected by treatment with 50 mm edta and egta , while it was increased by treatment with 3 - me 经注射鸡大肠杆菌后,文昌鱼体液中的凝集素对人b 、 o型红血细胞,兔、草鱼和蟾蜍红血细胞的凝集活性明显增强。
5.
The hemagglutinating activity of amphioxus skin mucus was decreased after heating at 60 for 30 min , and was almost lost upon heating at 100 for 30 min . the hemagglutinating activity of the skin mucus remained stable in the ph range from 6 to 10 , but it was inactivated at ph above 10 or below 6 . the hemagglutinating activity in the skin mucus is inhibited by d - galactose , which is same with the hemagglut inating activity of the humoral fluids of amphioxus 在60保温30分钟后,凝集素的凝集活性下降,在100保温30分钟后,凝集素凝集活性几乎完全消失;在ph6 - 10的范围内,凝集素的凝集活性稳定, ph大于10小于6的条件下,凝集素的凝集活性消失;文昌鱼体表粘液中的凝集素受d -半乳糖苷的抑制。
6.
The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times , gross lesions were observed in chicken embryo . the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin . to surprise , the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells . in addition , the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion , but it ' s gross lesion did n ' t resemble to those of field outbreaks 生物学特性:鸡胚尿囊液经离心、磷钨酸负染后,电镜观察该病毒为典型的冠状病毒;该毒株的第一代尿囊液对鸡胚无肉眼可见的致病作用,当继代到第5代后,胚体严重病变;病毒在鸡胚中随着接种时间的延长,其效价增高, 96h可达到48h的2倍;该毒株可在cef上生长,但不能形成明显的蚀斑;经1胰酶处理后可凝集鸡红细胞;鸡胚的第四代尿囊液病毒回归动物体,病死鸡肾脏呈典型的花斑肾,腺胃则未见肉眼可见的病变。
7.
The change of agglutinating activity , cd spectrum and fls of lra in different temperature , ph and different chemicals indicated that lra had partial hemagglutinating activity at ph2 . 0 ( 50 % ) , a temperature above 100 ( 60 % ) and after modified by n - bromosuccinimide ( mbs ) , the activity lost completely , modified by depc , the lra had a little activity , the other groups modified such as arg , tyr , glu , asp did n ' t effect the hemagglutinating activity of lra . the result indicated that trp residues were essential to the hemagglutinating activity and were involved in carbohydrate - binding site 研究不同温度、 ph和基团特异性化学修饰后lra凝血活性和促淋巴细胞有丝分裂的变化、圆二色谱和荧光光谱的变化,当温度达80以上时,活性开始下降,到100时活性有60 %保留:当ph为2时,活性保留50 % , ph为4一12对活性的影响不大;用nbs修饰trp后, t即的旦一叫睬基的破坏使活性完全丧失,表明trp对凝血活性是至关重要的, arg 、 tyr 、 glu 、 asp被修饰后, lra的凝血活性并未受到大的影响,但tyr修饰后lra的促有丝分裂活性降低