| 1. | The control of ib depended on completely the vaccination with the attenuated ib vaccine
该病目前无特效的治疗方法，主要使用ibv弱毒苗预防接种。 |
| 2. | There were deletion , insertion or mutation within the ibv isolates , the frontal 150nt showed the high variation
核苷酸序列前150bp的变异最大，不同毒株间存在插入、缺失及点突变等。 |
| 3. | Infectious bronchitis ( ib ) caused by infectious bronchitis virus ( ibv ) is an acute and highly contagious disease in chickens
传染性支气管炎( ib )是由传染性支气管炎病毒( ibv )引起的一种急性高度接触性鸡传染病。 |
| 4. | Soybean cotyledonary nodes were transformed with s gene of ibv via a . . tumefaciens - med ated transformation . the highest transformation rate of 6 . 7 % was detected in the present experiment
以子叶节为外植体，在抗性筛选培养基上大多白化死亡，有少数生长出芽，转化频率最高为6 . 7 |
| 5. | The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease . agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method
用三亲交配法将重组克隆导入根癌农杆菌，获得了含pbi121重组质粒的eha105农杆菌菌株。 |
| 6. | A pair of primer designed according to multi - sequence comparing results of published ibv sequences in genbank was used to amplify the nucleocapsid gene of 5 strains , the amplified rt - pcr product of all 5 strains is 1 . 2kb in length
对3个克隆质粒中的目的基因片段进行序列测定，结果表明该片段含有ibvn基因全序列，其结构基因全长1227bp ，编码409个氨基酸。 |
| 7. | The high specificity of dot - elisa was proved by the specific blocking test and the cross - reaction te st in which the diagnostic diaphragm did n ' t react with the positive serum against ibdv , ibv , eds - 76 , pox , ibv , iltv , salmonellosis jc . both the test within batch and the test among batches proved the method or the diagnostic diaphragm was stable . stored at 4c for at least 6 months , the diagnostic diaphragm ' s sensitivity and specificity did n ' t change
阻断试验和交叉试验表明快诊膜具有良好的特异性：不与马立克、法氏囊、鸡痘、鸡传支、鸡传喉、鸡减蛋综合症、鸡传鼻、鸡沙门氏菌等阳性血清反应。批内和批间重复性试验表明该法重复性良好。诊断膜片4至少可保存6个月，其特异性、灵敏性不变。 |
| 8. | 2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion -
S1基因：其全序列共1614bp (从起始密码子atg到s前体蛋白裂解位点) ，编码537个氨基酸，其氨基端有一编码18个氨基酸的信号肽序列，第12 13位氨基酸残基构成了信号肽的切割位点， 14 19位与111 124位氨基酸残基为s1蛋白的跨膜区域。 |
| 9. | 90 % of them were positive by pcr . southern blot analysis showed that ibv si gene had been integrated into genomic dna of potato and most transgenic plants have two copies of ibv si gene . northern blot analysis indicated that most transgenic plants could transcribe normally si gene of ibv though the level of transcription was different in the different tansgenic plants
对所得到的抗性植株进行分子生物学检测， 90的再生植株pcr检测为阳性； southernblot检测证明外源基因已整合到植物基因组内，大多数为二拷贝， northernblot分析表明多数转基因植株中外源基因能正常转录，但转录水平不一，也有外源基因发生基因沉默。 |
| 10. | The nucleotide sequences and deduced amino acid sequences of the m gene of 8 ibv isolates in china with the published sequences of reference strains in genbank were analyzed and compared , the results showed that the nucleotide sequences homogeneity between them is 87 . 1 % ~ 100 % , and the deduced amino acid sequences homogeneity is 88 . 1 % ~ 100 %
将m基因核苷酸序列和推导的氨基酸序列进行比较的结果表明ibv分离株彼此间的核苷酸同源性为87 . 1 - 100 ，其相应的氨基酸序列的同源性为88 . 1 - 100 。 |
