Westen immunoblotting with dl antibody and an approach detecting thylakiod phosphorylated proteins were used to check dl proteins of different forms , quantitive scranner to count abundances of proteins and fluorometry to detect the maxmin fluorescence ( fm ) of chlorophylls and influorescence of proteins 实验结果显示： ( 1 )光抑制处理使叶绿素a和叶绿素b发射荧光明显下降； ( 2 )光抑制处理时类囊体膜蛋白内源荧光并不发生明显变化。
2.
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting 本研究将含有n末端信号肽和c末端毒性区缺失的pap基因的表达载体ppic9k - p用sali酶切线性化后，通过电击转化整合p . pastorisgs115菌株细胞中。
3.
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation , c - ret phosphorylation and immunoblotting assays . the main results are as follows : 1 . expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity , the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s 纯化和复性后的重组gdnf蛋白，可显著增强pc12一gfral一ret工程细胞的存活和分化;对pc12一ret工程细胞没有任何作用;对pc12一gfral的存活和分化作用11中英文摘要显著强于对pc12一ret的作用，但也显著低于对pc12一gfral一ret细胞的作用。
