| 1. | Application of dot immunogold filtration assayfor detecting serum antibodies in clonorchiasis patients
斑点金免疫渗滤法检测华支睾吸虫病患者血清抗体的研究 |
| 2. | For further identification the existence of the extracellular ecbp21 , we used immunogold localization technology to study the subcellular localization of ecbp21
为了进一步确定ecbp21的胞外存在，本实验利用免疫金标定位技术，研究了ecbp21亚细胞定位状况。 |
| 3. | The results showed that distribution of 37 and 39kd peptides on the plasma membrane surface of sperm cells of maize with visible immunogold particles to be localized
免疫定位表明， 37kd , 39kd多肽是玉米精细胞质膜所特异的。有关此多肽的功能有待进一步研究。 |
| 4. | Change in the concentration of endogenous iaa was monitored with enzyme - linked immunosorbent assays elisa , and iaa localization in the tissues was demonstrated by means of immunogold - silver microscopy , with authors modification , during the regeneration after girdling in
L . vent .剥皮后维管组织再生过程中内源iaa浓度的变化，并用改进的免疫金银法测定了此过程中内源iaa在不同组织中的分布。 |
| 5. | The endocytosed albumin appears to undergo transcytosis in large esicles ( 500 nm in diameter ) , identified by immunogold staining of endogenous albumin by electron microscopy , to the basolateral membrane where the albumin is disgorged back to the peritubular blood supply
对内源性白蛋白进行免疫金染色然后在电镜下观察发现：这些重吸收的白蛋白以大的囊泡（直径500纳米）形式被跨细胞转运到肾小管基底膜侧，然后回吐到肾小管周血管。 |
| 6. | These results demonstrate that the dot - immunogold assay shows its practical value to monitor the brucella infections among the epidemic area and individuals with different occupations in the un - epdemic area , and this method can also be applied for the clinical investigation and epidemiological survey of brucellosis
结论滴金免疫测定法对布病疫区人群和非疫区重点职业人员开展布病监测具有实用价值，也可用于布病的临床检测和流行病学筛查。 |
| 7. | Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root
首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。 |
| 8. | 三 、 effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation . effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation were studied using immunogold electronmicroscopy
三、钙离子浓度对光暗适应罗氏沼虾感光细胞gq蛋白q亚基亚细胞定位的影响采用免疫胶体金电镜技术，研究光暗适应条件下不同钙离子浓度对罗氏沼虾感光细胞中gq蛋白。 |
