| 1. | The changes of expression of il - 1 receptor type i in the rat brain were observed by immunohistochemistry after lps or seb was administered intraperitoneally
一、在大鼠腹腔内给予细菌毒素lps或seb后，用免疫组织化学方法观察了型il - 1受体在脑内表达的变化。 |
| 2. | Female mice 4 - 6 weeks old were intraperitoneally injected with 10iu of pregnant mares serum gonadotropin ( pmsg ) , and 48h later with 10iu human chorionic gonadotropin ( hcg )
细胞周期进人m期以前， mpf的活性调节依赖cdc25和myt激酶之间的平衡，其平衡状态决定细胞周期进程。 |
| 3. | 6 . sulfated polysaccharides from brown seaweeds gs201 injected by img / d intraperitoneally for 7 days could promote da release from lesioned side of striatum in rats , but not from unlesioned side compared with ns treated rats
腹腔注射海洋褐藻多糖gs201lingd7d后， pd大鼠损毁侧sir的da释放量明显高于ns组大鼠损毁侧。两组大鼠健侧sirda释放的差异无统计学意义。 |
| 4. | Methods wt5bztwenty healthy male albino were intraperitoneally injected with urethane for basic anesthesia and injected with alphachloralose via ear marginal venous to maintain anesthesia , spontaneously ventilated via tracheotomy tube , with the instep record of breath and blood pressure
方法取健康雄性大耳白家兔20只，以乌拉坦腹腔注射行基础麻醉，氯醛酮耳缘静脉注射维持麻醉，气管插管置家兔于自发呼吸状态，同步记录呼吸血压。 |
| 5. | Comparing with controls , the amount and dyeing of immunopositive cells of il - 1 receptor type i in rat paraventricular nucleus of the hypothalamus and the supraoptic nucleus were significantly enhanced by intraperitoneally lps or seb injection ( po . 05 ) , and the dyeing areas and processes of positive cells were enlarged
与对照组比较， lps或seb腹腔注射后大鼠下丘脑室旁核和视上核中表达型il - 1受体的细胞数量显著增加，染色加深( p 0 . 05 ) 。阳性细胞的染色面积增大，突起增长。 |
| 6. | The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔内给予细菌内毒素lps或肠毒素seb ，用免疫组织化学方法观察了脾脏核增殖抗体及下丘脑室旁核和视上核中1型il 1受体的表达，并采用双标记技术观察了1型il刁受体阳性神经元和加压素及催产素表达的关系。 |
| 7. | Normal bn rats were vaccinated intraperitoneally with lctcv , ltcv and rpmi1640 culture buffer ( blank control ) respectively . one - way mixed lymphocyte reaction ( mlr ) , flow cytometric analysis of the apoptosis of peripheral t cell and the rate of cd4 / cd8 in peripheral blood were performed before and after vaccination . results mlr showed that the immune response capability of bn rat spleen cells in lctcv group were suppressed significantly after vaccination ( p < 0 . 01 )
于疫苗接种前和每次接种后第5天以被免疫bn大鼠的脾细胞作为反应细胞，以lou c大鼠的脾细胞作为刺激细胞(经丝裂霉素处理) ，进行单向混合淋巴细胞反应( mlr ) ，同时对外周血t细胞凋亡和cd _ 4 ~ + 、 cd _ 8 ~ + t细胞亚群进行流式细胞分析。 |
| 8. | In the first part , we observed the changes of expressions of type i receptor of il - 1 in the rat and mouse brain after intraperitoneally administration of different kinds and doses antigens respectively . in the second part including two experiments we cloned rat il - 6r ' s genes by pcr , expressed them in e . coli dh5 a and cos - 7 cell , and produced il - 6r ' s polyclonal antibody which is proved having more high liter , affinity and specificity 1
第一部分采用不同种类和剂量的抗原刺激，分两个实验，观察了大、小鼠脑内il - 1的i型受体表达的变化，探讨了脑内参与免疫调控的核团和细胞类型；第二部分分两个实验，运用pcr技术克隆了大鼠il - 6r的基因并进行了原核和真核表达，制备了特异性高、亲和力强和较高效价的抗il - 6r多克隆抗体，为进一步进行il - 6r的研究奠定了基础。 |
