To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process , a chimeric secretion form of calmodulin binding peptide , which contains a signal peptide , a calmodulin binding domain and a c - myc epitope was constructed . the chimeric gene was introduced into arabidopsis . it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin , which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant . by observing the potential phenotype change of apoplast calmodulin " antisense " plant , the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated 但这些多是采用生理学手段和药理学方法而得出的体外( invitro )实验结果，为了取得质外体cam在植物生长发育过程中发挥重要作用的invivo实验证据，根据动物中的一些研究方法，本实验设计并构建了带有信号肽、 cam结合肽( can小肽) 、 epitope ( c - myc )融合基因的载体，并将融合基因通过真空渗入法转入拟南芥，预期过表达的融合蛋白将会被分泌到细胞外并与质外体cam相结合，这样就会抑制质外体cam的功能，从而可以构建质外体cam的“反义”植株，通过观察质外体cam “反义植株”的表型改变，就可以推断质外体cam在植物生长发育过程中的功能。
