| 1. | An about 550 bps and an about 700 bps fragment could be seen after the vector pqe80l / dhfr / hpd - 3 was digested by bamh i + kpn i and by bamh i + hindiii r
经w亡sternblot证实，在大小分别约为3ikd和26kd的位置有两条清楚的蛋白带。 |
| 2. | The dutch operator kpn started to develop in germany mobile internet service based on ntt docomo s i - mode technology . such service will also be developed in holland and belgium
荷兰运营商kpnmobile在德国开展基于nttdocomoi - mode技术的移动互连网业务，相继还会在荷兰、比利时开展此项业务。 |
| 3. | Shenzhen - based huawei has also gained successes in western europe in recent years , announcing major sales and tie - ups with such major names as dutch carriers kpn telecom and britain ’ s bt and vodafone
现在,深圳华为已经在欧洲通信市场取得突破,分别与荷兰皇家电信、英国电信及英国沃达丰集团签订协议,成为它们的通信网络设备供应商。 |
| 4. | ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii
Sds一队ge分析可见重组原核表达载体pqe一80l / dhf侧hpd一3表达的目的蛋白带和对照质粒pqe一sol / dhfr表达的蛋白带，大小分别约为31kd和26kd 。 |
| 5. | The fusing gene was transformed into leaf tissue of tobacco cultivar nc89 . more than 100 plants were obtained . transgenes were confirmed to be inserted into tobacco genome of r0 generation by pcr and dot - blotting analysis
通过kpn位点与成熟番木瓜蛋白酶的cdna连成一个融合基因，并插入到pbi121的原gus位点，构建成nib -番木瓜蛋白酶融合基因的植物表达载体pnpa 。 |
| 6. | At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0软件，对所克隆的序列用相邻接点法( neighborjoining州j ） method ）进行多序列比对，分析其同源性，并构建基因进化树。 |
| 7. | Therefore , a - amylase has been used widely in many industrial fields , such as glucose production , beer brewing , fermentation trade , textile industry , and so on . the study on a - amylase is one of the most active fields in enzyme industrial fields . with the development of biotechnology , more and more scientists and researchers attempt to use dna shuffling technology to breed , screen and even " create " new a - amylase genes with higher activity and other new characters
设计引物时，上下游引物5 ’端分别添加了kpn和bamh酶切位点，克隆得到的基因片段和质粒载体都用kpn和bamh进行双酶切后，进行酶连、转化、筛选得到阳性重组子，经过蓝白斑验证、单酶切验证、双酶切验证、 pcr验证等一系列的验证后进行测序。 |
| 8. | 6 . southern blotting cotton genomic dna was digested with bglll , kpn i , hindlll , ecorvand ecor i respectively and hybridization was carried out with gharf probe labeled with dig . southern blotting analysis indicated that there were at least two copies encoding arf gene in the genome of cotton
Southernblot分析用bgl11 、 kpn 、 hindlllecorv 、 ecor五种限铝性内切酶分另酶切棉花洞a的基因组dna ，用地高辛标记的arf作为探针与之进行southern杂交c杂交结果显示， arp基因在陆地棉基因组中可能至少有两个拷贝。 |
| 9. | Two pairs of primers have been selected by using the software of designing , meantime , using one pare of primers reported as comparision , the best result of pcr was the primer got rid of the phya intron and brought kpn i and xbal i enzyme digestion site in its 5 ' - and 3 ' - . the length of the amplified fragment was about 1500bp
通过引物设计软件设计、分析、筛选出两对引物，用另一对已报道的引物作对照， pcr扩增结果是设计时没有去掉phya基因内含子的引物扩增效果最好，在该引物的上下游分别引入了kpn和xbal酶切位点，获得的特异性pcr产物长约1 . 5kb 。 |
