The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps . then it is used in the following experiments . the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment 本研究将含有人乳铁蛋白( hlf )基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 ’端调控序列的乳腺特异表达重组载体plncxhlf按常规方法提取、纯化、 pcr 、酶切鉴定后，用于细胞表达和精子介导转基因动物的研究。
