The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene , this intron contains donor sequence - gtatgc , lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene . this gene encodes a peptide of 467amino acid residues with molecular weight of 51 . 37kda , containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide 核苷酸序列分析表明, pcr扩增产物中包含有完整的phya基因,该基因全长1506bp ,其中包含一段长102bp的内含子,该内含子具有真菌植酸酶基因内含子的特征保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。该基因编码467个氨基酸,理论分子量为51 . 37kda ,其上有13个潜在的n -糖基化位点, n端19个氨基酸为信号肽序列,植酸酶活性位点序列( crvtfaqvlsrhgaryptdskgk )位于氨基酸序列的+ 71 + 93 。