This result showed that gus was transiently expressed in the foliage , and they were of laticifer - expression 因此认为gus基因在叶片成功的实现瞬时表达，而且具有乳管表达活性。
2.
Thus it ' s necessary to recombine the heterogeneous gene with the promoter of laticifer - expression gene , and then the heterogeneous gene will express in laticifers 为此需将外源基因与乳管表达基因的启动子连接，令其在乳管中表达。
3.
The results showed that the prime symptoms of the tpd include laticifer necrosis and cambium - like meristem formation that occurs around the necrotic laticifer in the inner bark 结果表明该病的基本症状是在树皮内层发生乳管坏死和在坏死的乳管周围产生形成层状分生组织。
4.
To identify its laticifer - expression character , two transient - expression vectors ( pirl , pbir2 ) were constructed . in the transient - expression vector , ref promoter sequence replaced camv35s promoter of pbi121 为验证其乳管表达活性，通过取代pbi121中间表达载体上的camv35s启动子，构建了两个含gus基因的瞬时表达载体pbir1 、 pbir2 。
5.
Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas . its latex is the unique source of crude rubber used in current industry . because of its special and important use , the rubber tree has been extensively planted in tropical areas . increase production is always the main target in rubber tree cultivation . since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant , not only the latex production had been increased largely , but also a new set of rubber tapping system had been established , leading to a series of economic benefit . owing to ethrel " s extensive application , its side effects had been found more and more obviously , such as tapping dry , speeding up senescence , shortening the life span of rubber tree etc . in order to overcome the side effects and increase production more availably , for a long time , people had carried out lots of research work on cell level , membrane level , physiology and biochemistry of laticifer contents . but the mech anism why ethrel increased latex production was not yet understood completely . this study had cloned the ethylene receptor gene ( efrl ) from rubber tree , and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level . the results were as follows : 1 但是，由于乙烯利应用的普及，乙烯利刺激割胶引起橡胶树发生死皮病及加速胶树衰老，缩短胶树寿命等副作用也越来越明显。为了克服这些副作用，使乙烯利能更有效地刺激增产，长期以来，人们在细胞水平、膜水平和乳管细胞内含物的生理生化层面上进行了大量的研究，但仍未完全了解乙烯对胶树的作用机制。本研究从分子水平入手，克隆橡胶树的乙烯受体基因( etr1 ) ，并在转录水平和蛋白质翻译水平上研究etr1基因在乳管细胞中的表达及与乙烯刺激的关系，取得了以下结果： 1
