Construction of leaf and leafstalk regeneration systems in two cherry rootstocks 樱桃砧木叶片和叶柄再生体系的构建
2.
So , the rank of inducing callus from easy to hard is cotyledon , hypocotyls , stem , leafstalk , radicel , endosperm , leaf and root . 3 诱导的难易分别为：根段、叶片、胚乳、胚根、叶柄、茎段、胚轴、子叶。
3.
The plant looked quite ordinary , much like alfalfa . it had four leaves on each leafstalk , two larger ones at the apex and two smaller ones lower down , and all of the leaves swayed rhythmically to music , especially folk music 其实她长得其貌不扬，有点像苜蓿草，一个叶柄上有四片叶子，叶柄顶端生了两片大叶，叶柄下端则生了两片形状相同的小叶。
4.
Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root 首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。
