A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed , which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs . ( 1 ) the tr antibody is immobilized via edc and nhs . comparing with the mpa sam method , this new approach shows greater frequency response and high sensitivity 应用edc和nhs使褐藻酸钠与胱胺偶合形成衍生褐藻酸钠作新的自组装材料，用于蛋白固定，应用这一新方法， ( 1 )实现了转铁蛋白抗体分子的固定，并对转铁蛋白参考血清进行了测定，该方法可在0 . 08 25 . 7 g / ml范围内对转铁蛋白进行测定。
