| 1. | Cytosolic free ca2 + and actin microfilaments play crucial roles in the regulation of pollen tube growth and they may interact with each other
作为调控花粉管极性生长的重要因子， ca ~ ( 2 + )和微丝在调控花粉管极性生长过程中可能存在相互作用。 |
| 2. | Actin , the major component of the dynamic microfilaments system , exists in nearly all eukaryotic cells , and plays an essential role in living activities
肌动蛋白在真核生物中广泛存在，由肌动蛋白参与形成的动态微丝骨架系统是细胞生命活动的基础。 |
| 3. | In primitive protoctisis , as in animals , the cell membrane ingrows under the control of a ring of actin microfilaments , and a furrow constricts the cell equator until it pinches the cell in two
在动物细胞中，胞质由肌动蛋白控制，在赤道面处向下凹沟，形成环状缢缩，最后缢缩断裂，细胞一分为二。 |
| 4. | The results showed that there was a great deal of microfilaments in root meristematic cells , there was a few microfilaments in cotton callus and abnormal plantlets ; by alignment of peac17 with cotton actin
因此推测，微丝在细胞中的这种分布可能与愈伤组织不能继续诱导出正常的植株而诱导出畸形苗有关。 |
| 5. | After dedifferentlation and redifferentiation , the explants had three fates : abnormal plantlets , death and healthy plants . the correlation between microfilaments , actin mrna and development had been reported
外植体通过诱导经历脱分化和再分化，最后大致有三种去处：诱导出畸形苗，死亡和诱导出正常植株。 |
| 6. | Using conventional and immunoelectron microscopic methods , the cytomixis in pollen mother cells of tobacco ( nicotiana tobacum ) and the distribution of actin microfilaments during this process were investigated in the present study
摘要应用普通电镜和酶联免疫电镜技术，研究了烟草花粉母细胞中的细胞融合现象及细胞融合过程中肌动蛋白微丝骨架的变化。 |
| 7. | All above showed that peacl preserved the polymerization activities of actin , and gfp fusion gave facilities for the study of microfilaments proper ties in vitro . monomer his - tagged peac1 - gfp could notably inhibit dnase i activities
上述结果表明，通过gfp的融合而在大肠杆菌中可溶性表达的peac1 ，不仅很好保持了肌动蛋白的聚合活性，而且gfp的荧光特性也方便了微丝体外特性的研究。 |
| 8. | Polymer his - tagged peac1 - gfp efficiently activated myosin mg - atpase activity , which indicated that peacl might take part in correlative living activities in vivo . moreover , this result provided experimental proof in vitro for fusing gfp to actin isoform directly to study the dynamics of microfilaments and its regulation in vivo . we prepared rabbit anti - pea actins polyclonal antibodies using peacl as antigen which being expressed and purified from prokaryotic cells , and the antibodies possessed better immunity activity to pea actins
通过肌动蛋白体外对dnase以及肌球蛋白atpase活性影响的研究，发现单体his - taggedpeac1 - gfp能显著抑制dnase活性，在肌动蛋白聚合条件下能有效激活肌球蛋白atpase活性，这一结果预示着peac1在体内可能参与相关的生命活动，为利用gfp直接与肌动蛋白异型体融合来研究体内微丝的动态变化及其调节提供了实验依据。 |
| 9. | Although fluorescence labeling method was widely used in the observation of microfilaments " dynamic distributions in vivo , all of the traditional methods have their limits . the use of gfp fusion and the preparation of new type of algae fluorescent probe will facilitate our further study on plant actins
荧光标记技术虽然广泛用于细胞内微丝骨架分布及其动态的研究，但传统的荧光标记方法用于植物肌动蛋白研究时都具有一定的局限性，利用gfp融合以及研制新型的藻荧光探针有助于对植物肌动蛋白的深入了解。 |
| 10. | We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus , healtny plants and abnormal plantlets . fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root , abnormal plantlets and callus that was unable to germinate into healthy plants
本研究选取正常棉花的根，已经培养了长时间不能分化出正常植株的棉花愈伤组织和棉花畸形苗为材料，采用石蜡切片，通过fitc -鬼笔环肽对材料微丝荧光染色，结合荧光显微镜观察。 |
