Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells , ros and the membrane potential of mitochondria during this process were measured . our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells , led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential , and caused senescence of 2bs cells rapidly . it supports the hypothesis of oxidative damage of senescence . moreover it is a better system for the study of the effects of ros during the process of replicative senescence 利用光学显微镜观察和酸性-半乳糖苷酶染色技术研究了高浓度葡萄糖对人二倍体成纤维细胞2bs细胞衰老进程的影响，并用流式细胞仪检测了此过程中活性氧和线粒体膜电位差的变化。结果表明： 200 mmol l的葡萄糖对2bs细胞有生长抑制作用，能引起活性氧含量的变化，导致线粒体膜电位差显著下降，并诱导了细胞的衰老。这为氧化损伤假说提供了新的证据，并为研究活性氧和复制衰老之间的关系提供了较好的体系。
