| 1. | The preliminary results showed that the crystals could be formed from the nifb " mofe protein
初步结果表明，这种晶体可能就是nifb ~ - mofe蛋白的晶体。 |
| 2. | The preliminary studies showed that they were a nifh or a nife mofe protein crystals
和dj ；的缺失femocoto铁蛋山的纯化、特吐鉴定及结晶研究的晶体。 |
| 3. | ( 4 ) h2 evolution by mofe protein with a - gln194 and citrate substitutions was inhibited by co at 32 % inhibition rate , comparing to 71 % of that for nifv - mofe protein
Co对nifv ~ - mofe蛋白放氢的最大抑制率为71 ， - gln ~ ( 194 )和柠檬酸双重替换使的co抑制固氮酶放氢的最大抑制率下降到31 。 |
| 4. | Like the crystal of nifb " mofe protein , the crystals of both a nifh protein and a nife mofe protein were obtained at the first in the world when the first experiment on the crystallization was done
对这两种突变种蛋白的特性和结晶进行了初步研究。在结晶实验时，也是一次就得到了国内外尚未报道的nifhmofe蛋白和nifemofe蛋白棕色闽氮菌突变种山。 ； 、 dj ； 。 |
| 5. | By using the similar separation and purification methods , a nifh and a nife mofe protein were obtained from azotobacter vinelandii mutants dj54 and dj35 . the results of the complement activity experiments showed that a nifh mofe could be activated by femoco
从棕色固氮菌突变种dj _ ( 54 )中得到了nifhmofe蛋白；并参与了棕色固氮菌突变种dj _ ( 35 )的nifemofe蛋白的分离纯化，所用方法与nifb ~ - mofe蛋白的分离纯化相似。 |
| 6. | Comparison of substrate - reduction properties of these altered mofe proteins with wt and nifv - mofe proteins showed that : ( 1 ) only the a - gln194 substitution did not affect the total electron to proton reduction and notably double altered mofe protein with substitution of citrate and a - lys190 only retained very poor proton reduction activity . ( 2 ) a - gln194 substitution made a more direct effect on n2 reduction then other two substitutions
四个突变体固氮酶、野生型和nifv ~ -固氮酶的mofe蛋白的底物还原特性比较表明： ( 1 ) - gln ~ ( 194 )替换不影响固氮酶还原质子时的总电子流；尤其引人注意的是，含有- lys ~ ( 190 )和柠檬酸双重替换的mofe蛋白几乎完全失去了质子还原的能力。 |
| 7. | Under suitable conditions , dark brown short rhombohedron crystals could be obtained from nifb mofe protein . both of the longest sides of the biggest crystal were o . lmm . the possibility and time of the formation of crystals , and number , size , quality , and shape of crystals obviously depended not only on the kinds and concentrations of the components in the crystalline solution , but also on the methods for crystallization and technical bias , etc
对nifb ~ - mofe蛋白的结晶及晶体生长进行了的研究，初步探讨了结晶溶液各组分的种类和浓度、结晶方法和实验操作等与能否出现晶体及晶体的数目、大小、质量、形状和出晶时间等的相互关系。 |
| 8. | When complemented with fe protein , the nifb mofe protein had hardly any ft - reducing activity , but could be significantly reactivated by femoco extracted from op mofe protein to a high proton - reducing activity , which was very close to the highest value of c2h2 - reducing activity report ed previously in other laboratorys
此粗提物可为用nmf抽提的opmofe蛋白的femoco激活，所得fe蛋白具有与opfe蛋白相似的互补活性，可使opmofe的比活性达到2192nmolc _ 2h _ 2 min mg蛋白。 |
| 9. | Whole cell c2d2 reduction by all four mutants comparing to wild type and ni / v mutant was also detected . the result showed that only single a - gln194 substitution did not perturb the stereospecificity of protonation of c2d2 . the above comparing results indicate that in mofe protein ( 1 ) a a - gln190 site and its association with homocitrate are important for the transfer of electron / proton to femoco , while a - his194 site and the homocitrate are independent in h2 evolution
对四个突变株细胞的c _ 2d _ 2还原特性及还原产物中反式-顺式- 1 ， 2 -二氘代乙炔的比例进行了测定并与野生型及nifv突变株相比较，结果表明只有- gln ~ ( 194 )替换不影响c _ 2d _ 2还原产物中反式-顺式- 1 ， 2 -二氘代乙炔的比例，即未改变固氮酶还原c _ 2h _ 2加氢的立体构型的专一性。 |
