The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively 经过在mym平板上的传代和抗性标记的筛选分别得到了同源双交换的菌株aved24和avecg 。
