After added into pc 12 cells medium , c . chinensis extract is confirmed as neuroactive components of inducing differentiation of pc 12 cells up to 50 mg / l 用不同浓度的菟丝子提取物作用大鼠嗜铬细胞瘤pc12细胞，研究发现菟丝子提取物能诱导pc12细胞分化。
2.
For the purpose of exploiting the affluent resource of traditional chinese medicines , rat pheochromocytoma pc 12 cells were used as a model to screen the neuroactive components from natural products . most of all , the effect of it on inducing the differentiation of pc 12 cells and / or execute the neuroprotective effect challenged by serum - deprivation and oxidative pressure , and its mechanism were studied in this paper 本文以利用我国丰富的天然药物资源为目的，用大鼠嗜铬神经瘤细胞pc12作为模型，筛选能模拟ngf的功能诱导pc12细胞分化、对神经细胞有一定保护作用的天然药物，并对其作用机制进行初步探索。
3.
According to the polarity , the residue was isolated with petroleum ether , chloroform , ethyl acetate , and n - buoh , respectively . the n - buoh fraction , confirmed as neuroactive component , was subjected to sephadex lh - 20 column chromatography to provide an extract fraction , as a buff powder , which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l 将菟丝子干粉用75乙醇浸泡后，减压蒸干后得到褐色浆状物，经石油醚、氯仿、乙酸乙酯、正丁醇萃取，经柱层析后，再用葡聚糖凝胶对有效成份进一步纯化，获得了菟丝子中能诱导pc12细胞分化的活性组分。
