Expression amount of rhbsag in the former was increased 60 % and 80 % respectively than the latter . antigenicity of pres2 of fusion protein was increased 8 times and 10 times in bmn cells and in pupa hemolymph respectively than nonfusion hbsag Bmpak - hbmp表达的融合m蛋白( pres2 + s )中， pres2抗原性显著提高，与bmpak - hbm表达的非融合蛋白相比，融合蛋白pres2抗原性在细胞中约提高了8倍，蛹中约提高了1o倍。
2.
The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting . the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized ) 用重组病毒bmpak - hbmp和bmpak - hbm [带有非融合乙肝表面抗原( pres2 + s )基因，为本实验构建]感染家蚕细胞及蛹，对两种病毒的表达产物用elisa进行了跟踪检测，结果表明，感染bmpak - hbmp的家蚕细胞及蛹中rhbsag的表达量分别为3
