| 1. | Local delivery of c - myc antisense oligodeoxynucleotide on vessel wall to prevent restenosis following ptca
术后再狭窄的防治作用机制 |
| 2. | Inhibition of antisense oligodeoxynucleotide for tissue factor to myocardial ischemic - reperfusion injury
组织因子反义寡脱氧核苷酸防治心肌缺血再灌注损伤的实验研究 |
| 3. | Effect of et - 1 antisense oligodeoxynucleotide on the hemodynamics of normal and experimental hypertensive rats
内皮素- 1前体基因反义寡核苷酸对正常和高血压大鼠血流动力学的影响 |
| 4. | The molecular mechanism of the apoptosis in ovarian cancer cells induced by antisense oligodeoxynucleotide of human telomerase reverse transcriptase
基因反义核酸诱导卵巢癌细胞凋亡的研究 |
| 5. | Effects of nuclear factor - b decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclumpsia
顺式诱骗寡脱氧核苷酸对子痫前期患者脐血清培养的脐动脉平滑肌细胞功能的影响 |
| 6. | Sodn oligodeoxynucleotide of pkb : 5cac atg gac gtc aaa gct tcc aa3 ' each base of both sodn and saodn oligodeoxynucleotide was sulfurated . methods 1 . superovulation , natural mating and eggs collection
Myt激酶的作用与cdc25相反，可以持续的使edcz的nlr14和t ”巧处于磷酸化状态而抑制mpf的活性，从而抑制细胞周期进程。 |
| 7. | Both cdc2 and cdc25 kinase activity were inhibi - ted by ly294002 and saodn oligodeoxynucleotide of pkb , which indicate that pkb may act as an initiator of g2 / m phase transition through the phosphoryla - tion of cdc25c at ser216
Pi3k介导的信号转导通路中， pi3k pkb是独立于其它通路的一个新发现的信号转导通路，且与ras ， g蛋白， pkc等有复杂的交互作用。 |
| 8. | B - actin primers : forward 5tcc tat cgg cct cct cct ac 3 " and reverse 5aga atg tag tcc gcc agg tc 3 " , designed for 200bps . saodn oligodeoxynucleotide of pkb : stac agc ctg aga gga cct acc tg3 "
在有丝分裂期， edc25经历216位ser磷酸化同时其水解酶活性增强，活化的mpf磷酸化并活化edc25 ，形成正反馈，结果进一步活化mpf前体，活化的mpf促进细胞周期g2 / m期转换。 |
| 9. | To make clear the hypothesis , a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study , respectively . we first studied the cellular localization of kvl . 2 and the co - localization of kvl . 2 protein and vegf receptors flk - 1 and flt - 1 , observed the effect of mcao on kvl . 2 expression and phosphrylation in the rat brain in vivo , then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl . 2 in sh - sy5y cells . finally , in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl . 2 phosphorylation , we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl . 2 phosphorylation
为了验证上述假设，本文分别在整体和离体水平，采用大脑中动脉缺血( middlecerebralarteryocclusion ， mcao )和体外氧?糖剥夺( hypoxiaandglucose - deprivation ， hgd )缺血模型，首先了解了kv1 . 2蛋白的细胞定位及与vegf受体flk - 1和flt - 1的共存情况，观察了整体mcao后缺血再灌不同时间大鼠脑内kv1 . 2蛋白的磷酸化水平变化，然后通过外源性给予vegf蛋白，在sh - sy5y细胞株上观察其对缺血细胞存活率及kv1 . 2蛋白磷酸化水平的影响，最后利用vegf反义脱氧寡核苷酸( oligodeoxynucleotide ， odn )特异阻断内源性vegf蛋白的表达，观察内源性vegf蛋白在缺血细胞损伤及调节kv1 . 2蛋白磷酸化中的作用，以进一步明确vegf缺血保护效应与其调节kv1 . 2蛋白磷酸化之间的关系。 |
| 10. | In this paper , a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied . in addition , we synthesized and analyzed a sequence - specific cleavage reagent , in which oligodeoxynucleotide was recognizing group . detailed work mentioned below were carried out : 1
本文在查阅大量文献的基础上，以1 ， 10 -邻菲咯啉和1 , 10 -邻菲咯啉- 5 , 6二酮为配体合成了一系列过渡金属配合物，详细研究了它们与pbr322dna的相互作用及其机理。 |
