| 1. | Clinical valuation and therapeutic target for her - 2 in overexpressed breast cancer
2过度表达的临床意义及其靶点治疗 |
| 2. | The cdna was subcloned into prokaryotic expression vector pet3d and overexpressed in e . coli bl21 ( de3 )
将该cdna插入原核表达载体pet3d并在大肠杆菌bl21 ( de3 )中过量表达。 |
| 3. | Rynai - w and xynb1 were all cloned into the plasmid vector ppic9 q , and were all overexpressed in pichia pastoris gs l 1 5
将xynb1序列克隆进酵母表达载体ppic9上，在毕赤酵母gs115中得到高效特异性表达。 |
| 4. | Intermediate - conductance - ca2 - activated k channels are overexpressed in endometrial cancer and involved in regulating proliferation of endometrial cancer cells
钙激活性中电导钾离子通道在子宫内膜癌组织中的表达及其在细胞增殖中的作用 |
| 5. | 3 ) western blotting of gas1p when mutant gpi17p overexpressed showed that there was accumulation of immature gas1p . this means that there is a defect in gpi - anchoing
3 ）蛋白质印迹分析表明，在突变gpi17p存在下，有未成熟gas1p的积累，表明存在蛋白质阶i化缺陷。 |
| 6. | Many scientists have introduced badh gene to plants and got transgenic plants which overexpressed badh , but the accumulations of glybetaine are too low to function effectively
许多研究者已得到了转badh基因植物，虽然转基因植物中badh的表达量提高，但是甘氨酸甜菜碱的积累浓度并不高。 |
| 7. | The expression products of pel gene was analysis by sds - page and olive oil plate , and the result indicated that pel gene was functionally overexpressed in pichia pastoris and up to 95 % of the secreted protein
表达蛋白pel - gs分泌至培养基中，分子量约28kd ，与pel大小相近，占分泌蛋白的95 。 |
| 8. | The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e . coli bl21 ( de3 ) . the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna编码区序列被克隆进原核表达载体pet - 30a ，并在大肠杆菌bl21 ( de3 )中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。 |
| 9. | For prokaryotic expression , the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ) . the gene was overexpressed in e . coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction
用pcr方法扩增番红花八氢番茄红素脱氢酶( pds )编码区序列，定向克隆到表达载体pet一艺1a ( + )上，获得重组质粒pet一21a ( + ) / cspds 。 |
| 10. | To obtain large amounts of appa phytase , the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter , respectively . the appa phytase was overexpressed in e . coli strain bl21 induced by lactose
为了大量表达appa植酸酶，我们将appa基因分别克隆至原核表达载体pet - 28a ( + )和杆状病毒转移载体pvl - 1393中，将其分别置于lac和polyhedrin启动子控制之下。 |
