Chinese translation for "pcr引物"

pcr primer

Related Translations:

pcr fingerprinting:  pcr指纹图

pcr克隆:  pcr cloning

pcr expression:  pcr表达

pcr表达:  pcr expression

pcr primer:  pcr引物

即时pcr:  real time pcrreal-time pcr

anchor pcr:  锚定pcr

pcr amplification:  pcr扩增聚合酶链反应扩增聚合酶链式反应扩增

suppression pcr:  要用到抑制pcr

touchdown pcr:  递减pcr降落pcr

Example Sentences:

	1.	According to the nucleotide sequence of selected antigen epitope , pcr primers supplemented with the ecor i and sal i sites were designed 根据选出的抗原表位区的核酸序列设计pcr引物，并于引物末端添加ecor和sa11酶切位点。
	2.	Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated 以从t - vector中酶切得到的tps1为模板，根据已知序列设计pcr引物，扩增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。
	3.	The results indicated that the pcr primers designed could distinguish between marine bacteria and terrestrial bacteria , and could be applied in distinguishing the psychrophiles . sixteen strains which could produce cold - active protease and chitinase were screened by selective medium and 初步的分析表明，所设计的pcr引物能够较好地区分海洋性细菌和陆源性细菌，并且可以用于嗜冷海洋细菌的区分。
	4.	Compared with the referred rab3a , the amplified rab3a beard five nonsense nucleotide mutations , but the deduced amino acid sequence from the nucleotide sequence were completely the same as the referred rab3a protein , which demonstrated that the cloned placenta rab3a was suitable for further study 与pcr引物设计的参照rab3a比较有5个核苷酸变异，与翻译的氨基酸序列完全一致。由此表明本实验获得的rab3a cdna可用于进一步研究。
	5.	Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3 各另外设计一对特异性pcr引物，导入适当限制性内切酶切点，以上述连有目的基因的克隆载体为模板，采用pcr方法扩增基因片段，获得长度约230bp的igf -和219bp的igf -成熟肽基因序列。
	6.	It encodes a protein of 865 amino acids with a signal peptide at the n - terminus , of which a polycystic - kidney - disease ( pkd ) - like domain , a triosephosphate - isomerase ( tim ) catalytic region , a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus 根据已知几丁质酶基因的dna保守序列，利用人工合成的pcr引物和cb101总dna ，扩增出一个约2 . 6kb的片段并克隆到pbluescript ks载体上。
	7.	The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos . all of the eight segments were amplified by rt - pcr , and the purified pcr products were done cycle sequencing with specific primers , furthermore , the sequencing products were purified and run gel on abi prism 377 dna sequencing machine . the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4 . 0 version ) and genedoc ( 2 . 3 version ) software 首先将实验用毒株在spf鸡胚中增殖，提取核酸，然后应用oligo ( 4 . 0版本)和gendoc ( 2 . 3版本)软件设计h9n2aiv所有8个基因片段特异的pcr引物及序列测定引物，通过rt - pcr方法扩增所有毒株的各个基因片段，纯化后用特异引物进行测序反应，反应产物纯化后在abiprism377dna序列分析仪上进行序列测定，然后应用wisconsinsequenceanalysispackage ( gcg ， 10 . 2版本) 、 phylogeneticanalysisusingparsimony ( paup ， 4 . 0版本)和treeview ( 1 . 5版本)软件进行序列的数据编辑、序列翻译、进化树绘制和遗传演化分析。
	8.	And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8 试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头，在接头序列和测定的f片段近tn5的序列上，设计了2对染色体爬行用的pcr引物，从b8菌株中提取基因组dna ， bamhi酶切，与染色体爬行接头连接，依次用2对引物进行pcr ，扩增出239bp产物，经克隆、测序，发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列，其余则为f片段728bp序列的一部分，为进一步进行染色体爬行，克隆和测定整个b和f基因，揭示阳菌株的拮抗分子机制提供了技术资料贮备。
	9.	In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli 根据已发表的iltvtk基因的核苷酸序列设计一对pcr引物，以增殖的两株iltv的dna为模板，分别对它们的tk基因进行pcr扩增。将回收的pcr产物连接到适当的质粒载体上，转化感受态大肠杆菌，通过筛选对iltvtk基因的阳性克隆进行扩增培养。

Similar Words：

"pcr克隆法" Chinese translation, "pcr克篓" Chinese translation, "pcr扩增" Chinese translation, "pcr任意引物" Chinese translation, "pcr限制酶谱法" Chinese translation, "pcr之友dna合成仪" Chinese translation, "pcr指纹图" Chinese translation, "pcs" Chinese translation, "pcs a set" Chinese translation, "pcs of staples" Chinese translation