Periplasmic electronic transport systems in bacteria 细菌中的间质电子转运系统
2.
In the second method , the protein is secreted across the cytoplasmic membrane using the signal peptide method and then two periplasmic helper proteins facilitate the movement of the protein across the outer membrane 第二种方法是用信号肽方法分泌到细胞膜外，然后两种周质帮助蛋白促进蛋白移出外膜。
3.
The purified enzyme had a specific activity of 68 . 6 u / mg protein . overproduction of pga was often limited by translocation and / or periplasmic processing steps , subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm 经deae - sepharosecl6b离子交换层析和butyl - sepharosecl4b疏水层析，即可得纯度提高20倍、比活为68 . 6u mg的青霉素g酰化酶，两步纯化的总得率达91 。
4.
Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well 通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。
