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Chinese translation for "pf4"

血小板因子Ⅳ
Example Sentences:
1.3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa . 2 after sds - page and densitometric scan analysis , the result show that expression level is 25 - 30 % of total bacterial proteins
山西医科大学2002届硕士学位论文一2dhsa pbv220 rhpf4经温控诱导表达后, sds page及凝胶密度扫描分析,表达产物占总国体蛋白的25 30 ,凝胶迁移特性与hpf4标准品相同。
2.Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully . 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr . after sds - page and densitometric scan analysis , the expression level of r hpf4 is 25 - 30 %
结论本研究运用pcr定点突变技术,完全去除了hpf4cdna基因3 ”端utrat富含区:改用大肠杆菌强串联终止密码子taaaataa ,成功构建高效表达克隆pbv220 rhpp4 。
3.Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点,设计合成一对特异引物,在pt7 - 7 - rpf4表达质粒的基础上,应用聚合酶链式反应( pcr )对其cdna进行改造,通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ,用快速pcr检测法、 dna测序分析,鉴定重组hpf4表达质粒的正确性。
4.Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody . our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4 . after purified and renatured , r hpf4 prepared by our methods has bioactivity like wide hpf4 . our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我们构建的rhpn原核高效表达系统经m page及凝胶密度扫描分析结果表明, rhpf4表达量占菌体总蛋白量的25 30 ,较原表达克隆pt7 7 rhpf4提高了近80倍,经快速高效的包涵体分高纯化工艺和复性工艺, rhpf4具有野生蛋白活性。
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