| 1. | Objective to explore the significance of estrogen receptor ( er ) and progesterone receptor ( pgr ) as a tumor - marker of liver cancer
目的研究雌、孕激素受体作为肝癌标志物的意义。 |
| 2. | Pcr products were purified with qiaquick pcr purification kit , followed by sequencing using the big dye terminator cycle sequencing ready reaction kits used with amplitaq dna polymerase fs
Pcr产物用pgr纯化试剂盒纯化之后，进行测序反应。最后标本在dna测序仪( 377 )电泳和分析。 |
| 3. | Conclusion the occurrence of plc probably has some relationship with reduced quantities of pgr , and the quantities of er and pgr have no relationship with the recurrence of plc after operation
结论肝癌的发生可能与肝癌组织中孕激素受体含量的降低有关，雌、孕激素受体与肝癌术后的复发没有关系。 |
| 4. | Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna提取试剂盒按说明提取，提取的rna逆转录合成cdna ， cdna为pgr反应扩增的模板，特异性的引物是针对各个基因片段设计的。 |
| 5. | Methods receptor binding assay ( rba ) was adopted to measure the distribution of pgr and er in liver cancer and tissue in its vicinity in 63 patients with primary liver cancer ( plc ) undergone laparotomy and in non - tumor liver tissue in 7 cases with gastric carcinoma
方法采用放射性配基结合分析法( rba )测定了63例行手术切除的肝癌组织、癌周组织及7例非肝癌肝组织的雌、孕激素受体的含量并结合临床资料进行统计分析。 |
| 6. | Sweetpotato pollens killed by u . v . didn ' t sprout ; 2 . normal pollens sprouted ; 3 . pollens of 5x mixed with recognition pollens attached and sprouted much ; 4 . in the negative - cross , sweetpotato pollens attached and sprouted much on the stigma of 5x ; 5 . in the possitive - cross without recognition pollen , 5x pollens few attached and sprouted ; 6 . in the treatment of pgr ( twice ) , globular - embryo observed on 15 days after pollination ; 7 . ovule obtained by intercross germinated on the medium ; 8 . plantlet from intercross ovule grew on the medium ; 9 . seeds obtained by opening pollination ; 10 . tubers of hybrids from 5x crossed by sweetpotatos for two generations
紫外线杀死的甘薯花粉在亲和柱头上不萌发； 2 .未经紫外线处理的甘薯花粉在柱头上正常萌发； 3 .在蒙导花粉作用下，五倍体的花粉在甘薯柱头上大量附着和萌发； 4 .反交组合甘薯花粉在五倍体柱头上大量附着和萌发； 5 .正交组合无蒙导花粉时五倍体花粉少量附着和萌发； 6 .生长调节剂二次处理后，授粉后15天所见的球形胚； 7 .杂交胚珠在培养基上萌发； 8 .杂交胚珠培养成苗； 9 .放任授粉收获的大量种子； 10 .五倍体与甘薯杂交两代产生的后代群体的结薯性。 |
| 7. | ( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 )
( 1 )黄瓜子叶节植株再生建立东北农业大学理学硕士学位论文一通过对比三种外植体（于叶、子叶节、下胚轴）的分化率，得出最佳外植体类型为子叶节；通过探讨pgr （ naa 、 6 ba ）浓度和配比对不定芽分化的影响，得出最佳芽诱导培养基为：卜1叭6 。 |
