Bacillus subtilis can hydrolyze phytate , release phosphorus and improve phosphorus nutrition of plants and animals by secreting phytases 摘要枯草芽孢杆菌通能过分泌植酸酶分解植酸，释放磷元素，促进动植物磷的吸收。
2.
Phytases are hydrolytic enzymes that initiate the release of phosphate from phytate , the major phosphorus ( p ) form in animal feeds of plant origin . phytases are found naturally in microorganisms and plants 植酸是植物性饲料中磷的主要贮存形式，植酸酶是一种能将植酸水解并释放出无机磷的酶，它广泛存在于微生物与植物中。
3.
Phytases are a class of phosphatases that can catalyze the hydrolysis of phytate into myo - inositol and phosphate . it can relieve anti - nutrition of phytate and improve the nutritional value of animal feed from plant . the study on phytase , especially acidic phytase produced by micro - organism , has drawn much attention in home and abroard 研究表明，饲料中添加植酸酶可使其中磷利用率提高60 ，粪便中磷排出量减少40 ，还可以提高饲料转化率，改善动物的生产性能，因此对提高养殖业的经济效益及环境保护均具十分重要意义。
4.
Phytases catalyze the hydrolysis of phytic acid or phytate ( myo - inositol hexakisphosphate ) to inositol and inorganic phosphate . their roles in eliminating anti - nutritional effects of phytate in plant fodder or foods , in increasing the efficiencies of protein and various microelement utilizations in the animal body and the animal productivities , in reducing the amount of phosphorus in animal excretions and thus reducing environmental pollutions , etc , have been the research focuses domestically and internationally 植酸酶是一类能催化植酸及植酸盐水解成肌醇和无机磷酸盐的酶，它具有解除植物性饲料(或食品)中植酸的抗营养作用、提高机体对蛋白质及多种微量元素的利用率、促进生长发育、提高动物生产性能、减少粪便中磷的排放量、降低磷对环境的污染等多种功能，因而受到国内外的广泛关注。
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Monogastric animals , such as pigs and poultry , are not able to utilize phytate phosphorus efficiently , since they have only low levels of phytase activity in their digestive tracts , phytate also acts as an antinutritional agent in monogastric animals by chelating various microelements needed by the animal 由于单胃动物如猪、鸡等的消化道中植酸酶活性很低，导致它们无法有效利用植酸磷。同时，植酸也是一种抗营养因子，因为它能螯合许多动物生长所必须的微量元素。
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Escherichia coli phytase appa is an alternative enzyme with performance advantages over the conventional a . niger enzyme . the appa phytase displays several favorable characteristics : an acidic ph optimum close to the physiological ph range of the stomach of pigs and chickens , higher affinity to sodium phytate , greater resistance to pepsin than the commercially available a . niger phya , and higher catalytic efficiency for phytate than that of all other known phytases 大肠杆菌来源的appa植酸酶是一种新型的植酸酶，与常规的黑曲霉植酸酶相比具有许多优点，如与猪、鸡等畜禽胃的ph环境更加适应，与底物植酸更强的亲合性以及对消化道蛋白酶更强的抵抗性。
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The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium . after being isolated and purified , these strains were inoculated into fermented medium , shaking in 28 c at 220r / min for 5 days , then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2 . 5 . the result showed there were 24 strains with higher enzymatic activities among the 102 strains , after the rescreening , 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase , of which , enzymatic activity of the strain 14 was the highest , reaching 53 . 86u / ml , and it was preliminarilly identified as aspergillus . niger , then numbered as aspergillus . niger 14 用植酸钙选择性平板培养基从土样中筛选出了102株能产透明圈的菌株，经分离纯化后，接入液体发酵培养基， 28 、 220r min发酵5天，在37 、 ph2 . 5条件下用钒钼酸铵法测定其所产植酸酶的活力，结果显示，酶活较高的有24株，经再次摇瓶复筛后，酶活大于15u ml且产酶性能稳定的共有7株，其中以14 ~ #菌株的酶活最高，可达53 . 86u ml ，经初步鉴定为黑曲霉，编号为aspergillus . niger14 ~ # 。
8.
In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate . among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing . after fermented for 5days at 28 c and shaking at 220r / min , the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2 . 5 or ph5 . 5 主要结果如下： 1植酸酶高产菌株的筛选利用植酸钙选择性子板培养基从土样中筛选出102株酸性植酸酶产生菌，从中挑选出透明圈较大的菌株32株，经分离纯化后分别进行摇瓶复筛， 28 、 220r / min发酵5天后，在37 、 ph2 . 5或ph5 . 5条件下用钒钼酸铵法检测其酶活，结果发现有3株菌产酶活性较高且产酶性能较为稳定。
9.
Phytase is a kind of enzymes which can catalyze the hydrolysis of phytate into myo - inositol and phosphate . it can relieve anti - nutrition of phytate and improve the nutritional value of animal feed from plant , the study on phytase , especially acidic phytase produced by micro - organism , is paid much attention by scientists in our country and abroard 植酸酶是催化植酸及植酸盐水解成肌醇和无机磷酸盐的一类酶，它能够解除动物植物性饲料中植酸的抗营养作用，提高植物性饲料的营养价值，因而受到国内外广泛关注，尤其是微生物所产生的酸性植酸酶。
