| 1. | Cpges mrna signal was maily located in oocyte cytoplasm and declined at 48 h after pmsg treatment
在pmsg处理后的卵泡颗粒细胞中，几乎检测不到cpges免疫染色。 |
| 2. | However , the tyrosine - phosphorylation of stat3 was weak after pmsg treatment for 48 h followed by hcg treatment
但pmsg处理48小时后用hcg处理则使stat3的酪氨酸磷酸化减弱。 |
| 3. | There was a decline in cpges mrna expression 48 h after pmsg treatment . this decline went on until 11 h after hcg treatment
Pmsg注射48h时， cpgesmrna的表达下降，而且这种表达的下降一直持续到hcg注射后11h 。 |
| 4. | Mpges immunostaining became slightly weaker 12h after pmsg treatment . the highest level of mpges immunostaining was detected 3 h after hcg treatment
在hcg注射后3小时， mpges的表达呈现峰值，随后，蛋白的表达下降。 |
| 5. | Female mice 4 - 6 weeks old were intraperitoneally injected with 10iu of pregnant mares serum gonadotropin ( pmsg ) , and 48h later with 10iu human chorionic gonadotropin ( hcg )
细胞周期进人m期以前， mpf的活性调节依赖cdc25和myt激酶之间的平衡，其平衡状态决定细胞周期进程。 |
| 6. | The expression , tyrosine - phosphorylation and nuclear translocation of stat3 were increased in the luminal and glandular epithelium of immature mice by pmsg treatments . hcg treatment also stimulated the tyrosine - phosphorylation of stat3 in the nuclei of luminal epithelium
用pmsg处理性未成熟的小鼠，可刺激子宫腔上皮和腺上皮中stat3的表达，并促进子宫腔上皮和腺上皮中的stat3发生酪氨酸磷酸化及核转位。 |
| 7. | The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop . firstly , female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse , the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo . then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro
母鼠在注射hcg后与公鼠同笼交配，并在注射hcg后不同时间(从18h到26h ) 、不同方法(体内法和体外法)获取胚胎，研究获取原核胚的最佳时间；在此基础上获得具有第二极体原核胚，然后对原核胚进行显微操作，去除雌原核并注入第二极体，探讨第二极体内遗传物质支持胚胎发育的潜力；同时利用dna特异性染料h33342对原核胚进行染色，显示并比较原核和第二极体内的dna遗传物质。 |
| 8. | In this study , we showed that the pdk inhibitor ly294002 regulated the cellular localization of materials 1 . kunming mice were supplied from the department of laboratory animals , china medical university 2 . pregnant mare serum gonadotropin ( pmsg ) and human chorionic gona - dotropin ( hcg ) were obtained from tianjin huafu biological products research institute and shanghai products research insititute , respectively
为了研究在小鼠受精卵中pkc 、 pkb是否也通过p21蛋白参与调控g2 m转换，本实验应用了pma和ly294002作为pkc 、 pkb的抑制剂，研究它们对小鼠受精卵中p21蛋白表达和定位，进一步探讨可能的调控机制。 |
| 9. | Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e . metck darmstadt methods superovulation and collection of eggs for superovulation , female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ) , and after 46 - 48 hours with human chorionic ginadotropin . ( hcg ) . one - cell fertilized eggs were collected on the next day from oviduct of females
取4一5周龄成熟雌性昆明系小白鼠，腹腔注射pmsg (孕马血清促性腺激素) 10iu ， 46一48小时后腹腔注射hcg (人绒毛膜促性腺激素) 1oiu ，将注射hcg后的雌鼠与8周以上的成熟雄鼠合笼交配，次日检察阴栓，将查到阴栓的雌鼠处死，取输卵管于mz培养液中，解剖镜下撕开壶腹，释放细胞团，然后用300林岁nil透明质酸酶消化去除颗粒细胞，口控吸管将卵细胞在m :中反复清洗，然后置于孵箱中，根据时间点收集g2期细胞。 |
