| 1. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ，在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 2. | 2 . a new framework , named information entropy model of sliding window ( iemsw ) , was proposed to effectively analyze the structural information contained in the 3 ' - utr sequences around the polyadenylation site . 3
为有效地分析水稻3 - utr序列剪切位点上下游序列中的信息结构，提出了一个新的分析框架，即dna序列的滑动窗口信息熵模型。 |
| 3. | And then a 378bp acci fragment in pbtk2 . 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni , a eukaryotic expression plasmid
在此基础上，进一步用tthlll酶切后，补平插入带有sv40启动子的大肠杆菌?半乳糖苷酶报一告基因( lacz ) ，获得通用转移载体pltk - uni 。 |
| 4. | Deduced amino acid sequence of s1 , s2 , pvin were also highly homologous each other ( 98 % , 99 % in each case ) . the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector , pbi121 and pev2 , from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct . the recombinant plasmids were called pbs2 , pev2s 1 . respectively
用bamhi和saci同时酶切ps2 ( s2表示来自雷司令的芪合酶基因) 、 ps1 ( s1表示来自粉红玫瑰的芪合酶基因)以及pbi121 、 pev2 ，使得s2 、 s1分别插入替代pbi121 、 pev2中的gus基因，构建成植物表达载体pbs2 、 pev2s1 ， pbs2中含camv35s组成型启动子，使s2基因能在番茄植株的各个部位表达； pev2s1则含有果实特异性启动子tfp2 ，使s1基因只在番茄果实中表达。 |
| 5. | Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20 . 7kd , 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail . 3
棉花arf基因的序列分析核苷酸序列分析表明， gharf的全长为828bp ，其中含有一个从131bp到676bp间的546bp构成的一个完整开放阅读框，它编码181个氨基酸、分子量为20 . 7kd的蛋白质。 |
| 6. | Our research demonstrates that rd - pcr is an effective technique for constructing cdna fragments libraries in bacteria , which can greatly decrease the redundancy of cdna libraries , leading to reduction of time and labor in our further endeavor to prepare cdna microarrays . we assessed changes in rna levels after exposure to heat shock using the microarray and found the polyadenylation level of mrna significantly decreased after exposure to heat shock
实验研究发现限制性显示pcr技术可以很好地用于细菌的poly ( a )化mrna的限制性cdna文库的构建，巧妙地解决了由于细菌mrnapoly ( a )化位点的高度多态性，用常规方法构建原核cdna文库所遇到的文库大量重复冗余的难题。 |
| 7. | But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found . the sites of polyadenylation of bacterial mrna are diverse , including the 3 ' ends of primary transcripts , the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions , and sites within the coding regions of mrna degradation products
细菌mrna多聚腺苷酸化的位点多种多样，包括初级转录产物的3 ’末端， 3 ’端非翻译区和顺反子间区的内切酶加工位点及mrna降解产物的编码区内，其腺苷酸化相对无特异性、无选择性。 |
| 8. | It has been known that polyadenylation of 3 " end of mrna in prokaryotes is different from that of eukaryotes . the poly ( a ) tracts in bacteria are generally short , ranging from 15 - 60 adenylates residues and associated with only 2 - 60 % of the molecule of a given mrna species . mrna polyadenylation of eukaryotes always occurs in the untranslated region , 10 - 30 nucleotides downstream of the hexanucleotide sequence aauaaa
现在普遍认为细菌许多mrna3 ’端存在多聚腺苷酸尾，但其长度通常较短，大约在15 - 60腺苷酸残基范围，且对某一mrna而言，仅有2 - 60的分子被多聚腺苷酸化。 |
