| 1. | The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3
同时还将表达运载蛋白dhfr的基因单独连接于表达载体pqe - 80l ，得到对照质粒pqe - 80l dhfr 。 |
| 2. | Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe
对重组pmd一18一t克隆载体及pqe一30表达载体双酶切，提取tctp基因和pqe一30空载体并使二者重组，然后转化m15 ，挑取阳 |
| 3. | ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii
Sds一队ge分析可见重组原核表达载体pqe一80l / dhf侧hpd一3表达的目的蛋白带和对照质粒pqe一sol / dhfr表达的蛋白带，大小分别约为31kd和26kd 。 |
| 4. | The high at content of the native gene is very difficult to express , we designed a new he gene by reverse the codon used for e . coli and yeast , then , the he gene was cloned into the expression vector pqe - 60
本研究尝试了其保护性抗原基因在大肠杆菌及酵母中的表达。天然bont a的hc片段基因中at含量高达76 ，其通过同义密码子置换人工合成hc段基因，将at含量降到57 。 |
| 5. | 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ，经酶切鉴定，得到重组原核表达载体pqe - 80l dhfr h d - 3 。 |
