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Chinese translation for "recombinase"

重组酶
Example Sentences:
1.In the present study , we develop an efficient method to be used to examine recombination efficiency of transgenic plantlet . two constructs , one containing cre recombinase under the control of
对cre在转基因个体中介导的重组效率进行了研究。构建了含有
2.To abolish transcription efficiency affected by copy number or integration site in chromosome , all the vectors were integrated into frt site by co - transfecting with an flp recombinase producing plasmid
应用w七stemblot对其中的20个克隆进行检测,选出一个caspase一3表达量最低的细胞克隆a6 。
3.After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut
经pcr检测后,有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带,表明发生了替换反应;另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用,剪切了gfp基因,从而只扩增出了载体序列和一个lox位点的约500bp的条带。
4.This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1 . 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites , loxp and lox2272 , would facilitate cre - recombinase mediated cassette exchange ( rmce ) , which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
该载体的特点是:具有可以调控外源基因在乳腺中特异表达的牛-酪蛋白基因5 `端侧翼区和包括第一外显子及内含子在内的5 `端调控区;将人血清白蛋白cdna准确地置于牛-酪蛋白基因第二外显子中的翻译起始密码子atg之后,而且没有增加额外的序列和使人血清白蛋白cdna移码;引入标记基因gfp ,便于在胚胎期鉴定阳性胚胎,减少受体;引入cre lox重组系统: ( ? )标记基因gfp的两端的两个loxp位点可以在表达载体整合到基因组后,删除标记基因; ( ? )余下的一个loxp位点可以和前面的lox2272位点组成cre重组酶介导的盒式交换系统。
Similar Words:
"recombinant vaccine" Chinese translation, "recombinant virus" Chinese translation, "recombinant yeast cells" Chinese translation, "recombinant-vector vaccine" Chinese translation, "recombinants" Chinese translation, "recombination" Chinese translation, "recombination action" Chinese translation, "recombination activating gene" Chinese translation, "recombination analysis" Chinese translation, "recombination and repair" Chinese translation