Each segment of dna thus synthesized is called a replicon . 这样合成的每一片段DNA称为复制子。
2.
Each segment of dna thus synthesized is called a replicon 这样合成的每一片段dna称为复制子。
3.
For vector pbmb1205 and pbmb1205r , the plasmid original replicon ori44 was come from b . thuringiensis subsp 它们的构建为今后构建高毒力基因工程菌提供了很好的材料。
4.
Kurstaki strain hd263 . the plasmid original replicon ori1030 of vector pbmb1206 and pbmb1206r was cloned from b . thuringiensis subsp . kurstaki strain hd73 该类载体的构建在国内尚无人报道，国外也没有成熟的实用性好的解离载体的报道。
5.
Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites 解离载体的构建和性能利用来源于不同质粒上的质粒复制起始区ori44和ori1030构建了4个解离载体。
6.
Therefore , the 4 . 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm , ) and resulted in a cloned strain bl - 3 . this strain could express only 130kda protein 它不仅对苏云金芽胞杆菌工程菌的构建具有现实意义，而且对其它基因高表达研究将产生积极影响，具有一定的理论意义和潜在的应用价值
7.
A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally 进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。
8.
Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker 本文将粪产碱杆菌青霉素g酰化酶( afpga )基因构建表达载体pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc启动子、青霉素g酰化酶基因、 rrnb转录终止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷贝复制子；而psmlfpga含有四环素抗性基因和p15a中拷贝复制子。
