| 1. | A complete cdna of 1047 bp was obtained by means of 5 ' - race ( 5 ' - rapid amplification of cdna end ) techniques using gene specific primer p1a5 - 1
Rtpcr结果证明它在苞片中强烈表达而在处于同时期的叶片中几乎检测不到其表达。 |
| 2. | Furthermore , we found the mutagenesis relies on the alteration on gene expression profile induced by mnng treatment ; and also , the expression of dna polymerase p ( pol p ) was proved increased after mnng treatment
这种不依赖于dna损伤的非定标性突变随后被证明依赖于mn ’ ng引起的细胞内基因表达的变化。进一步我们用rtpcr技术证明dna聚合酶e （ p 。 lp ）的表达在mn ’ ng处理后显著升高。 |
| 3. | Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts . the aberrant expression of sh2a gene in some cancers was found . it suggests that sh2a gene relates to tumors
Rtpcr及northern印迹杂交发现sh人在多种组织中有表?二?达，有3个转录本，而且在癌及癌旁组织的比较中发现，肿瘤组织中表达较高，初步证明与肿瘤的发生有关。 |
| 4. | Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique . homologous analysis of the gene was researched by blast . then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr
正常组织及肿瘤组织实验方法利用捕获的外显于进行blast同源性分析，之后采用外显于拼接技术克隆新基因，利用rtpcr及northern印迹杂交检测该基因的组织表达情况，同时以p 《 ctin作内对照。 |
| 5. | Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l .
采用阿r和rtpcr技术，从甘蓝和油菜基因组和柱头总则a中扩增获得了thlz基因，基因组中得到的基因大约为900hp和850hp ， cdna大约为477hp 。在thlz基因中首次发现了两个内含于，并且甘蓝和油菜thlz基因的内含于序列同源性低于ich ，并且大小也有差异。 |
| 6. | This suggests that mxmybl represents a single copy gene in the genome of mains xiaojinensis . 8 expression pattern was analysed by northern blot and rt - pcr . the results showed that mxmybl mrna was expressed in root and leaf and it was strengthened expression by the treatment of fe - deficiency for three days in roots especially
8 、 northcm杂交结合rtpcr的方法对mxmybl基因在小金海棠中的表达模式进行了分析，结果如下： mxmyb在根系和叶片中均表达，缺铁处理可以加强mxmybl在根系中的表达，尤其是缺铁3天的根系表达量最强。 |
| 7. | The n - terminal nucleotides 47 - 420 of the guangxi apmv - 1 isolates of different poultry species origin were amplified and sequenced . the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
研究对鸡、鹅、鸽三种禽源apmv刁广西分离株f基因n与前段进行了rtpcr扩增及核茸酸序列测定，并用基因分析软件dnastar进行分析并与已发表的其它参考毒株进行比较。 |
| 8. | The results of gst - pulldown and yeast two - hybrid showed kyot2 interact with them respectively in and yeast and vitro . 3 using the yeast two - hybrid system , we isolated a novel protein , kbp ( kyot binding protein ) . three types of cdnas were identified by rt - pcr and were designated as kbp1 , kbp2 , kbp3 , respectively
通过rtpcr我们获得了kbp全长的cdna ，并发现它存在三种不同的剪接体ep 、 kbpz和ep3 ，同时通过计算机比较还获得了它的基因组全长，这三种cdna和基囵组序列已经在g bank上登录于邱。 |
| 9. | According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然后根据dsrna设计原则，结合nssb基因的序列特征，借助生物信息学软件设计了针对nssb基因的sirnas ，并交由公司化学合成；电穿孔法转染上述稳定转染的细胞克隆，同时分别以非特异的sirnas转染组和空白转染组为对照， dapi染色后通过荧光显微镜和内标化rtpcr检测，初步证实了化学合成的sirnas可以特异阻断nssb基因的表达。 |
| 10. | The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr . sequence analysis of the gene was described in this papel the amino acid sequence , as derived from the nucleotide sequence of a cdna clone , had high homology with other subtilisin - like serine protease of nematogenous fimgi
与其他丝氨酸蛋白酶基因序列比较表明，与其他线虫卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性较高，而与捕食线虫真菌arthrobotrysoligospora同源性较低( 45 ) 。 |
