Optimization of conditions for extracting crude polysaccharide from hygrophorus russula 淡红蜡伞子实体水溶性粗多糖提取方法探索
2.
Russula subnigricans hongo grows fast after shower in the later summer and early autumn when it was hot . they emerged in the forest stand in which crown density was 0 . 9 or so . soil ph was 5 . 75 , and fagaceae species dominated 每年在夏末秋初高温阵雨之后为发生盛期。发生地林间郁闭度0 . 9左右，土壤ph值5 . 75 ，其共生树种以壳斗科为主。
3.
Emphasis is placed on taxonomic discussion on the taxa . families including two or more genera and genera including three or more spec ies have keys facilitating retreiving . 43 species in 15 genus of 8 families ectomycorrhizal fungi have been found in those two forests 在已查明的114种伞菌中，共发现外生菌根菌8科， 15属， 43种，占总数的33 . 1 ，种类最多的是红菇属russula和丝膜菌属cortinarius 。
4.
The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis . the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands . apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells 4 .用电泳技术研究亚稀褶黑菇粗毒液诱导小白鼠肝肾细胞凋亡，小白鼠亚稀褶黑菇抽提液中毒后，肝肾细胞. dna经琼脂糖凝胶电泳出现180一200bp整数倍的ona梯形带， 19 . 09 / l一28 . 59 / l范围内，亚稀褶黑菇提取液诱导肝肾细胞凋亡表现出时间和剂量依赖性
5.
The biological characteristics and toxicity of russula subnigricans hongo was studied for the first time from ecology and morphologic characteristics and histology , the orthogonal experiment of the optimum culture condition , the analysis of components , apoptosis of the cells from little white rat liver and kidney induced by extract of russula subnigricans hongo , to the histopathologic changes observation of little white rat liver and kidney through ecological observation , light microscopy and scanning electron microscopy , reversed - phase high performance liquid chromatography , agarose gel electrophoresis , transmission electron micioscopy . the result showed as below : based on ecological observation of russula subnigricans hongo , its ecological environment was investigated in order to simulate its ecological environment when they are cultivated 利用菌种分离技术、光镜技术、电镜技术、高效液相色谱技术、毒理实验技术、电泳方法等对亚稀褶黑菇( russulasubnigricanshongo )的生物学特性和毒性机理进行了研究，主要包括以下内容：亚稀褶黑菇的生态学和组织学研究、菌种分离培养、扫描电镜观察、成分分析、粗毒液诱导小自鼠肝肾细胞凋亡，小白鼠中毒后肝肾细胞透射电镜观察，研究结果如下： 1
6.
Russula subnigricans hongo was studied with light microscopy and scanning electron microscopy techniques . the result showed that russula subnigricans hongo is a kind of ecotomycorrhizal fungi , russula subnigricans hongo emerged and developed in the relatively steady ecological environment where average temperature , humidity and soil water content were 24 ~ 32 , 50 ~ 80 % and 30 % respectively . among the three environmental factors , soil water content was the leadig factor 通过野外生态调查和利用扫描电镜对亚稀褶黑菇进行了生态学和组织学研究。亚稀褶黑菇是一种外生菌根菌，在相对稳定的生态环境条件下，平均温度24 32 ，相对湿度50 80 ，降雨量(土壤含水量) 30以上，均有亚稀褶黑菇发生，湿度是主导因子。
