Among numerous strategies for c - terminal protein sequencing ( iso ) thiocyanate method established by schlack - kumpf in 1926 appears to be a promising approach because of the similarity to the edman degradation in mechanism 纵观蛋白质c端测序方法的发展，由于化学法中的(异)硫氰酸法反应机理与edman降解十分类似，方法，所以是最有前景的方法。
2.
However , due to the poor reactive carboxyl group of c - terminus , it is difficult to derivatize peptides topeptidyl t hiohydantoins for c - terminus , and the schlack - kumpf degradation based on thiohydantoin procedure has not yet been developed to such an efficient method as edman degradation 然而，由于c端羧基的化学性质不活泼，致使schlack - kumpf降解法的研究进展缓慢。但通过近十年的研究表明该方法是很有希望成为常规c端顺序测定的方法。
3.
( iso ) thiocyanate method is also called schlack - kumpf degradation . we spend much time on the derivatizing reagent which is extremely crucial to this method . four derivatizing reagents that seemed theoretically practical have been introduced , after thoroughly invest igation of them , two of which are practical and can derivatizing the amino acids to their corresponding thiohydantoins : ( 1 ) tributylsilyl isothiocyanate ( tbus - itc ) ; ( 2 ) triphenylgermanium isothiocyanate ( tpge - itc ) (异)硫氰酸法又称为schlack - kumpf降解，本文在关键的偶联试剂方面进行了不懈的努力，先后对理论上有可能成为偶联试剂的四种化学试剂进行了摸索，最后确定了其中两种是可行的： ( 1 )三丁基硅异硫氰酸酯( tbus - itc ) ； ( 2 )三苯基锗异硫氰酸酯( tpge - itc ) 。
