| 1. | Mt - were prepared from the rabbit liver induced by zinc after isolation and purification . they were demetalled in acidic condition , then applied sephadex g - 25 to gain apomts free of metals
从锌诱导的家兔肝脏中分离纯化得到两种亚型的金属硫蛋白mts ： zn |
| 2. | An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化，得到抑制黑曲霉生长的单一组分。 |
| 3. | The purification of this halocin was achieved by combination of tangential flow filtration ( tff ) , sephadex g50 and deae - sepharose chromatography . the n - terminal amino acid sequence was also determined by edman degradation
Halc8对大多数的极端嗜盐古菌(包括部分嗜盐碱古菌)有抑制作用，但对细菌则无明显的抑制作用。 |
| 4. | In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 |
| 5. | 4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100 , g50 ) . at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod
因而不管从第一次的纯化来看，还是从文献上报道的来看，本实验的串联柱层析是较为合理有效的一种生产方法，值得推广。 |
| 6. | 3 . the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100 . the specific activity of the purified enzyme was increased by 37 . 9 folds over crude extract , and the recovery yield was 8 . 9 %
以粗酶液为起始，经硫酸铵分级沉淀、 sephadexg - 100凝胶过滤后，分离纯化了该酶，纯化倍数约为37 . 9 ，酶活力回收率8 . 9 。 |
| 7. | The crude cellulases from liquid fermentation of b - 6 and ass . 3711 were isolated and purified by ( nh4 ) so4 precipitation , sephadex g - 100 and deae - sepharose cl 6b column chromatography . the cmcase components were purified and some of their physical and chemical properties were studied
本文将液体发酵的酶液经硫酸铵分级沉淀、柱层析后得电泳纯cmcase组分，并对as3 . 3711和b - 6来源的cmcase酶解动力学和理化性质作了比较研究。 |
| 8. | The isolation of crude cu , zn - sod can be started with organic solvents ( ethanol - chloroform ) in the classical manner . then we take two different methods : the first one is that chromatography is carried out on sephadex g100 , g50 at room temperature with ph7 . 6 , 0
纯化后的两种样品经聚丙烯酰胺凝胶电泳鉴定和酶活性测定，结果表明：两种方法分离纯化的sod纯度高，活力也高，说明后者在前者的基础上发展起来后，更趋于合理。 |
| 9. | An antifungal protein , named as b16 , was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate , desalt and gel filtration on sephadex g - 75 , which with molecular weight at about 30 - 40 kd on the basis of sds - page
菌株041381发酵上清液经70饱和度硫酸铵沉淀，透析脱盐， sephadexg - 75凝胶过滤层柱，得到活性物质b16 。以sds - page胶为基础进行电泳分析， b16的分子量为30 - 40kd 。 |
| 10. | One is a combination of ion exchange chromatography on q sepharose ff , hydrophobic interaction chromatography on phenyl hp , gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q . the other is a combination of ion exchange chromatography on q sepharose ff , two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially
上清液中重组蝗蛇毒类激血酶的纯化是通过设计的不同分离纯化方法组合进行的，并对每一种组合进行了活力与纯度及口收率的评价，结果表明两种组合方式都具有一定的实用性和可操作性。 |
