Ddrt - pcr method was exploited to study the differential gene expression between immature siliques of arabidopsis wide - type and ast mutant 采用ddrt - pcr的策略，分析野生型与突变型植株未成熟角果中基因表达的差异。
2.
This procedure was simple , time - saving , highly sensitive and reproducible . based on the improved procedure , nearly 16 , 000 cdna fragments were analyzed between the immature siliques of the wide type and ast mutant , and 28 differential cdna fragments were screened 采用这个改良的的方法，分析了拟南芥野生型和ast突变型植株未成熟角果中16 ， 000个cdna扩增产物条带，从中筛选出28个差异条带。
3.
Here we found g proteins also function in leaf , silique development and the yield of pollen microspore . we observed several traits or characters in the offsprings of gpal , agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type , whereas the lamina length , petiole length and rosette diameter is smaller than the wild type , the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type , and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines 实验中我们跟踪观察了多代异三聚体g蛋白a亚基超表达转基因植株及a ， p亚基缺失突变体的表型特征，发现突变体的叶片宽度大于对应的野生型，叶片长度，叶柄长度及莲座直径小于野生型，而超表达植株的上述某些特征与突变体相反; gp时突变体的长角果长度，花梗柄部长度大于野生型，而超表达ga植株种英则略小于对照; gpal突变体长角果尖端未出现咭乙i突变体的特征: gpal ，口gbl突变体花粉生成量大于野生型，而超表达ga植株的花粉生成量则略小于对照。
