| 1. | Effects of cultural conditions on growth and sporulation of monilinia laxa aderh ruhl . honey
培养条件对桃褐腐病菌生长和产孢能力的影响 |
| 2. | Effect of inertia carrier culture on sporulation yields and conidia viability of paecilomyces fumosoroseus
惰性载体对玫烟色拟青霉产孢和孢子活力的影响 |
| 3. | However it is susceptible to protease ( s ) and cannot express in a large amount in the sporulation phase of natural occurring strain of b . s
但它在野生型菌株中的表达量低，易被蛋白酶降解失活。 |
| 4. | The result shows that the life cycle of all the species comprises a unicellular amoeboid or swarm cell stage , a multinucleate plasmodium stage and a sporulation stage
结果表明，生活循环均包括单核的黏变形体或游动胞、多核的营养体原质团以及形成孢子阶段。 |
| 5. | Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cryld - lacz , when sodium acetate acted as carbon source , cryid - lacz could keep high , stable expression in different hosts
琥珀酸钠作碳源时导致芽胞形成提前， cry1d - lacz融合基因表达也随之提前。而以乙酸钠作碳源时，在不同亚种的菌株中都能高水平、持续稳定地表达。 |
| 6. | Three different batches of broomcorn millet cultures obtained under uniform conditions in millet processing , initial inocula level , and incubation were found not significantly differing in sporulation capacity . on non - nutritional agar plates , temperature significantly affected sporulaiton
本研究用白碳黑、硅藻土和变色硅胶对新鲜黍米培养物进行处理并在5和10下保存，均导致其快速丧失产抱能力。 |
| 7. | Protoplast regeneration , treatment of mutagenesis and other unknown factors can increase the genomic inherent instability , which usually affects the following five phenotypic traits : sporulation , pigmentation , colony morphology , antibiotic production and antibiotic resistance
基因组的不稳定性通常会影响菌株的以下五种表型特征：产孢，产素，菌落形态，抗生素产生水平以及对相应抗生素的抗性水平。 |
| 8. | Northern blotting analysis showed that cryld mrna was more stable than crylab mrna , however , cryld mrna formed later 3h and less 3 . 7 times than crylab mrna during the mid - phase of sporulation . moreover , crylab mrna could keep stable and large amount during the post - phase of sporulation . this suggested that difference of transcriptional efficiency and initiate time might be main reason for the difference of expression of crylab and cry id
结果表明：基因cry1dmrna的形成比基因cry1ab的mrna滞后3小时，且基因cry1d形成mrna的量很低，产生过程很平稳，在芽胞形成中期比cry1abmrna低3 . 7倍； cry1abmrna含量在芽胞形成前期高于后期，在后期仍能大量持续稳定地转录。 |
| 9. | 3 . the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ) , p3a was spliced with the cry1c gene , then inserted into the shuttle vector pht304 , and then recombinated plasmid pbmb827 was obtained . after transferring pbmb827 into strain ybt - 1520 , it was surprising that the transformants had almost no potency against all lepidopteran larvae tested
3非依赖芽胞形成icp的cry3a启动子( p _ ( 3a ) )对野生菌株特性的影响带p _ ( 3a )和cry1c基因的重组质粒pbmb827转入ybt - 1520 ，转化子对所测昆虫的毒力下降非常明显，芽胞和晶体也很难脱落。 |
| 10. | The recombinant b . thuringiensis subsp . israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant . it was found that b - pmpx2 strain remained stable toxicity , whatever during vegetative phase or sporulation phase , which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b . sphaericus
同时，将来源于苏云金芽孢杆菌以色列亚种( b . thuringiensissubsp . israelensis , b . t . i )的cytlaa基因和p20伴侣蛋白基因克隆连接到质粒pmt9中，并转化到苏云金芽孢杆菌无晶体型中得到重组转化子b - pmpx2 ，电镜观察发现重组转化子b - pmpx2形成一菱形晶体。 |
